Light BYTES – September 2020 : George McNamara wins Competition with SPECTRA Light Engine®

SPECTRA Light Engine® Takes the Win Highlighting smFISH

In celebration of Earth Day Lumencor launched its annual Light Microscopy Imaging Competition to highlight Lumencor’s commitment to manufacturing bright, clean, and mercury-free light engines. The tallies are in and we are happy to announce the winners. As always, we are impressed with the breadth and skill it took to capture each of the images submitted in this years competition. For more information on how Lumencor light engines can help you in your research, please contact us.

 

1st Place – George McNamara and Lauren Blake
Johns Hopkins School of Medicine, Baltimore, MD
Light Engine: SPECTRA light engine®

5-plex mouse embryonic fibroblasts expressing MS2-tagged beta-actin mRNA. Red Halo-JF549-NLS-MCP, Green Atto594 POLR2A mRNA FISH, Blue DAPI, Cyan Cy5 beta- actin-MS2 mRNA FISH, Magenta Alexa Fluor 488-anti-DDX6 immunofluorescence. Microscope details at http://confocal.jhu.edu/ current-equipment/fishscope. Specimen preparation by Lauren Blake, Prof. Bin Wu’s lab (JHU Biophysics). Imaging by George McNamara, Ross Fluorescence Imaging Center.

 

 

2nd Place – Ariel Waldman
Independent Researcher, Antartica
Light Engine: SOLA SM light engine®

A nostoc discovered in Antarctica autofluorescing under TRITC excitation. This nostoc was found inside a microbial mat in the Dry Valleys of Antarctica. A nostoc is a genus of cyanobacteria with beaded filaments intricately woven inside a gelatinous pouch. This image highlights the structure of the beaded filaments and their scaffolding within the gelatinous pouch.

 

 

 

 

3rd Place – Tejeshwar Rao
University of Alabama, Birmingham, AL
Light Engine: SOLA SE light engine®

 

Cos-7 cells spread on a tension gauge tether (TGT) surface and imaged on a Nikon Ti2 eclipse microscope using a Lumencor light engine and a turret wheel with different excitation and emission filter cubes – The RICM image (panel 1) was taken by removing the emission filter from the path. Cell tension indicated by TGT probe opening (panel 2) and paxillin staining (panel 3).


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