Application Bibliography
| Application | Area Product | Reference | Date |
|---|---|---|---|
Simultaneous visualization of 16 small (62–73 kb) DNA loci in G1 phase hTERT RPE-1 cells using single-molecule FISH | Fluorescence in Situ Hybridization (FISH) SPECTRA Light Engine | Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH. Sci Data (2022) 9:47 A Mota, M Schweitzer, M Bienko et al. | 2022 |
Identification of Parkinson’s disease-specific cellular phenotypes from high-content imaging datasets | High-Content Screening SPECTRA X Light Engine | 2022 | |
Hybridization-based in situ sequencing (HybISS) via direct RNA detection for identifying low abundance transcripts in mouse brain sections | Transcriptomics SPECTRA X Light Engine | Direct RNA targeted in situ sequencing for transcriptomic profiling in tissue. Sci Rep (2022) 12:7976 H Lee, SM Salas, M Nilsson et al. | 2022 |
Optogenetic induction of telomere coalescence | Optogenetics SPECTRA X Light Engine | Compartmentalization of telomeres through DNA- scaffolded phase separation. Dev Cell (2022) 57:277–290 A Jack, Y Kim, CP Brangwynne et al. | 2022 |
Quantitative immunofluorescence analysis of dystrophin expression in muscle biopsy tissue | Diagnostics SOLA Light Engine | 2022 | |
Imaging of labeled mRNA transcripts for validation of probe hybridization | Transcriptomics SPECTRA X Light Engine | 2022 | |
One-photon photostimulation of fast-acting, high-conductance ChroME2.0 opsins | Optogenetics SPECTRA X Light Engine | High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks. Neuron (2022) 110:1–17 S. Sridharan, MA Gajowa, H Adesnik et al. | 2022 |
Evaluation of the photovoltaic effect in molybdenum disulfide (MoS2) semiconductor flakes | Materials Science SOLA Light Engine | Mechanical stress modulation of resistance in MoS2 junctions. Nano Lett (2022) 22:1047–1052 P Chaudhary, H Lu, A Gruverman | 2022 |
340/380 nm fura-2 imaging of dysregulated Ca2+ homeostasis in mouse fibroblasts | Live Cell Microscopy RETRA Light Engine | 2022 | |
Super-resolution radial fluctuation (SRRF) microscopy of chromatin | Superresolution Microscopy SPECTRA Light Engine | 2022 | |
Quantitative immunofluorescence analysis of tropomyosin expression in mesenchymal stromal/stem cells (MSCs) | Immunofluorescence Microscopy SPECTRA X Light Engine | 2021 | |
Image-based identification of CRISPR-induced phenotypes | High-Content Screening SPECTRA X Light Engine | High-content imaging-based pooled CRISPR screens in mammalian cells. J Cell Biol (2021) 220:e202008158 X Yan, N Stuurman, RD Vale et al. | 2021 |
Single-molecule RNA fluorescence in situ hybridization (smFISH) in Saccharomyces cerevisiae | Transcriptomics SPECTRA X Light Engine | 2021 | |
Reflection interference contrast microscopy (RICM) of unlabeled microtubules. | Biophysics SOLA Light Engine | Self-repair protects microtubules from destruction by molecular motors. Nat Mater (2021) 20:883–891 S Triclin, D Inoue, M Théry et al. | 2021 |
Detection of streptavidin-phycoerythrin (SAPE) fluorescence linked to hybridization of exosomal microRNAs on hydrogel microparticles | Diagnostics SOLA Light Engine | 2021 | |
DNA FISH chromatin tracing correlated with immunofluorescence detection of epigenetic markers in IMR-90 cells | Transcriptomics CELESTA Light Engine | 2021 | |
High-throughput neurite outgrowth analysis using organotypic explant cultures | High-Content Screening SPECTRA X Light Engine | 2021 | |
Two-color immunofluorescence detection of SARS-CoV-2 spike protein localization on HEK293T cell membranes | Immunofluorescence Microscopy SOLA Light Engine | Rapid characterization of spike variants via mammalian cell surface display. Mol Cell (2021) 81:5099–5111 K Javanmardi, CW Chou, IJ Finkelstein et al. | 2021 |
Ultraviolet photoactivation of NP-caged isopropyl ß-D-1-thiogalactopyranoside (IPTG) | Optogenetics SOLA Light Engine | (Optochemical) control of synthetic microbial coculture interactions on a microcolony level. ACS Synth Biol (2021) 10:1308–1319 A Burmeister, Q Akhtar, A Grünberger et al. | 2021 |
Automated whole-slide scanning FISH analysis on formalin fixed, paraffin embedded (FFPE) specimens | Fluorescence in Situ Hybridization (FISH) SPECTRA Light Engine | Optimized workflow for digitalized FISH analysis in pathology. Diagn Pathol (2021) 16:42 V Chea, V Pleiner, M Tinguely et al. | 2021 |
Immunofluorescence detection of PI(4,5)P2 and lipid phosphatase synaptojanin1 in cultured mouse astrocytes | Immunofluorescence Microscopy SPECTRA X Light Engine | 2021 | |
Imaging of lipid droplets in Drosophila larvae | Confocal Microscopy CELESTA Light Engine | Arc1 and the microbiota together modulate growth and metabolic traits in Drosophila. Development (2021) 148:dev195222 SA Keith, C Bishop, BM McCartney et al. | 2021 |
Rapid fluorescence-based immunoassay for detection of viral pathogens including SARS-CoV-2 | Diagnostics SOLA Light Engine | 2021 | |
Imaging of photoactivated calcein NVOC in live slices of mouse small intestine, lung, spleen, and brain tissues | Transcriptomics SPECTRA X Light Engine | Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes. Nat Commun (2021) 12:4995 AS Genshaft, CGK Ziegler, AK Shalek et al. | 2021 |
Visualization of double strand break repair in single Escherichia coli cells by high-throughput multicolor fluorescence microscopy | Live Cell Microscopy SPECTRA Light Engine | RecA finds homologous DNA by reduced dimensionality search. Nature J Wiktor, AH Gynnå, J Elf et al. | 2021 |