Application Bibliography

Application	Area Product	Reference	Date
Photoactivation of rose bengal dye (575 nm, 1 mW) to induce localized ischemic damage in mouse brain	Optogenetics RETRA Light Engine	Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice. Cell Rep (2021) 36:109427 (opens in new window) Y Abe, S Kwon, KF Tanaka et al.	2021
Timelapse imaging of intracellular Ca²⁺, mitochondrial membrane potential and cytosolic lactate	Live Cell Microscopy SOLA Light Engine	CDK2 limits the highly energetic secretory program of mature β cells by restricting PEP cycle-dependent K _ATP channel closure. Cell Rep (2021) 34:108690 (opens in new window) SM Sdao, T Ho, MJ Merrins et al.	2021
Quantitation of Alexa Fluor 647-labeled DNA binding to GFP-tagged transcription factors	Microfluidics SOLA Light Engine	High-throughput affinity measurements of transcription factor and DNA mutations reveal affinity and specificity determinants. Cell Syst (2021) 12:112–127 (opens in new window) AK Aditham, CJ Markin, PM Fordyce et al.	2021
Immunofluorescence detection of SARS-CoV-2 viral nucleocapsid in Vero E6 cells	Immunofluorescence Microscopy SPECTRA Light Engine	Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors. PLoS Pathog (2021) 17:e1009165 (opens in new window) M Mor, M Werbner, NT Freund et al.	2021
Immunofluorescence detection of coronavirus receptors on Vero-E6 and BHK-R cells	Immunofluorescence Microscopy SPECTRA X Light Engine	Targeting polyamines inhibits coronavirus infection by reducing cellular attachment and entry. ACS Infect Dis (2021) 7:1423 ~1432 (opens in new window) MR Firpo, V Mastrodomenico, BC Mounce et al.	2021
Immunofluorescence microscopy detection of EpCAM+/CD45-/DAPI+ circulating tumor cells (CTC)	Diagnostics SPECTRA Light Engine	An automatic platform based on nanostructured microfluidic chip for isolating and identification of circulating tumor cells. Micromachines (2021) 12:473 (opens in new window) HJ Jou, LY Chou, HT Hsu et al.	2021
6-hour time-lapse imaging of GFP-labeled Golgi and Hoechst 33342-stained nuclei in live human umbilical vein endothelial cells	Microfluidics SPECTRA X Light Engine	Endothelial cell polarization and orientation to flow in a novel microfluidic multimodal shear stress generator. Lab Chip (2020) 20:4373–4390 (opens in new window) UM Sonmez, YW Cheng, LA Davidson et al.	2020
MERFISH imaging of chromatin organization and transcriptional activity	Transcriptomics CELESTA Light Engine	Genome-scale imaging of the 3D organization and transcriptional activity of chromatin. Cell (2020) 182:1641~1659 (opens in new window) JH Su, P Zheng, X Zhuang et al.	2020
Ca²⁺ imaging of transgenic mice expressing GCaMP6f in cortical excitatory neurons	Multimode Imaging SPECTRA X Light Engine	Simultaneous cortex-wide fluorescence Ca²⁺ imaging and whole-brain fMRI. Nat Methods (2020) 17:1262~1271 (opens in new window) EMR Lake, X Ge, RT Constable et al.	2020
TIRF microscopy assay for detection of glycosphingolipid vesicle binding to surface-immobilized human norovirus VLPs	Bioengineering SPECTRA Light Engine	Histo-blood group antigens of glycosphingolipids predict susceptibility of human intestinal enteroids to norovirus infection. J Biol Chem (2020) 295:15974-15987 (opens in new window) I Rimkute, K Thorsteinsson, G Larson et al.	2020
Photoswitching of Dendra2 fluorescent protein for phenotypic annotation of single cells	High-Content Screening SPECTRA X Light Engine	High-throughput, microscope-based sorting to dissect cellular heterogeneity. Mol Syst Biol (2020) 16:e9442 (opens in new window) N Hasle, A Cooke, DM Fowler et al.	2020
100 fps single particle tracking of 0.2 and 0.1 µm beads in hyaluronic acid hydrogels	Bioengineering SPECTRA X Light Engine	Particle diffusion in extracellular hydrogels. Soft Matter (2020) 16:1366~1376 (opens in new window) F Burla F, T Sentjabrskaja, GH Koenderink et al.	2020
High-throughput 3D imaging of cell-induced matrix deformations using X-Light V2 spinning disk system	Confocal Microscopy SPECTRA Light Engine	Mechanophenotyping of 3D multicellular clusters using displacement arrays of rendered tractions. Proc Natl Acad Sci U S A (2020) 117:5655~5663 (opens in new window) SE Leggett, M Patel, IY Wong et al.	2020
Quantitation of NRF2 and MAFG expression in murine astrocytes using multiplexed error-robust FISH (MERFISH)	Fluorescence in Situ Hybridization (FISH) CELESTA Light Engine	MAFG-driven astrocytes promote CNS inflammation. Nature (2020) 578:593~599 (opens in new window) MA Wheeler, IC Clark, FJ Quintana et al.	2020
Identification of CRISPR interference (CRISPRi) genotypes following phenotypic screening	High-Content Screening SOLA Light Engine	Time-resolved imaging-based CRISPRi screening. Nat Methods (2020) 17:86~92 (opens in new window) D Camsund, MJ Lawson, J Elf et al.	2020
Long-term (12-24 hour) time-lapse imaging of GFP, YFP and RFP	High-Content Screening SPECTRA X Light Engine	Isolating live cells after high-throughput, long-term, time-lapse microscopy. Nat Methods (2020) 17:93~100 (opens in new window) S Luro, L Potvin-Trottier, J Paulsson et al.	2020
Quantitative immunofluorescence profiling of three-dimensional organoids formed by human mesenchymal stromal cells	Immunofluorescence Microscopy SPECTRA X Light Engine	Mapping the structure and biological functions within mesenchymal bodies using microfluidics. Sci Adv (2020) 6:eaaw7853 (opens in new window) S Sart, RFX Tomasi, CN Baroud et al.	2020
Particle streak velocimetry for characterization of high-speed pulsatile fluid flow	Microfluidics SOLA Light Engine	Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction. Lab Chip (2020) 20:3744~3756 (opens in new window) T Qian, DA Gil, MC Skala et al.	2020
Detection of IgG secretion from murine B cells compartmentalized in 0.05 nanoliter droplets	Immunofluorescence Microscopy SOLA Light Engine	The Quantitative Assessment of the secreted IgG repertoire after recall to evaluate the quality of immunizations. J Immunol (2020) 205:1176~1184 (opens in new window) K Eyer, C Castrillon, J Baudry et al.	2020
Immunfluorescence detection of cardiovascular remodeling markers in FFPE rat heart tissue sections	Immunofluorescence Microscopy SPECTRA Light Engine	Statins reverse postpartum cardiovascular dysfunction in a rat model of preeclampsia. Hypertension (2020) 75:202~210 (opens in new window) K Krker, JM O'Driscoll, N Haase et al.	2020
12-color brightfield illumination for high-speed multiplexed biomarker detection	Spectral Imaging SPECTRA Light Engine	Brightfield multiplex immunohistochemistry with multispectral imaging. Lab Invest (2020) 100:1124~1136 (opens in new window) LE Morrison, MR Lefever, DR Bauer et al.	2020
Fluorescence endoscopic detection of colorectal neoplastic lesions	Intravital Imaging SPECTRA Light Engine	Topical application of Chlorin e6-PVP (Ce6-PVP) for improved endoscopic detection of neoplastic lesions in a murine colitis-associated cancer model. Sci Rep (2020) 10:13129 (opens in new window) I Ganzleben, M Hohmann, MJ Waldner et al.	2020
Mesoscopic Ca2+ imaging in GCaMP6 transgenic mouse brain	Intravital Imaging SPECTRA X Light Engine	Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits. Nat Methods (2020) 17:107~113 (opens in new window) D Barson, AS Hamodi, MJ Higley et al.	2020
Excitation of EuTTA (377/50 nm) and fluo-4 (485/20 nm) for measurement of intracellular temperature and Ca2+ in electrically stimulated hippocampal neurons	Live Cell Microscopy SPECTRA Light Engine	Single-cell temperature mapping with fluorescent thermometer nanosheets. J Gen Physiol (2020) 152:e201912469 (opens in new window) K Oyama, M Gotoh, N Fukuda et al.	2020
High-speed (20 fps) widefield 3D microscopy of the neuronal endoplasmic reticulum	Live Cell Microscopy SPECTRA X Light Engine	Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells. Sci Adv (2020) 6:eaay9572 (opens in new window) SD Carter, CM Hampton, Z Freyberg et al.	2020