Application Bibliography

Application	Area Product	Reference	Date
550 nm illumination of hybrid perovskite films for characterization of degradation pathways	Materials Science RETRA Light Engine	Water-accelerated photooxidation of CH₃NH₃PbI₃ perovskite. J Am Chem Soc (2022) 144:5552–5561 (opens in new window) TD Siegler, WA Dunlap-Shohl, HW Hillhouse et al.	2022
Imaging genotype and phenotype at single-cell resolution by sequencing cellular perturbation identity in situ.	High-Content Screening CELESTA Light Engine, SOLA Light Engine	Pooled genetic perturbation screens with image-based phenotypes. Nat Protoc (2022) 17:476–512 (opens in new window) D Feldman, L Funk, PC Blainey et al.	2022
Imaging of spectrally-encoded lanthanide nanoparticles (LNP)	Microfluidics SOLA Light Engine	A bead-based method for high-throughput mapping of the sequence- and force-dependence of T cell activation. Nat Methods (2022) 19:1295–1305 (opens in new window) Y Feng, X Zhao, PM Fordyce et al.	2022
Simultaneous visualization of 16 small (62–73 kb) DNA loci in G1 phase hTERT RPE-1 cells using single-molecule FISH	Fluorescence in Situ Hybridization (FISH) SPECTRA Light Engine	Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH. Sci Data (2022) 9:47 (opens in new window) A Mota, M Schweitzer, M Bienko et al.	2022
Identification of Parkinson’s disease-specific cellular phenotypes from high-content imaging datasets	High-Content Screening SPECTRA X Light Engine	Integrating deep learning and unbiased automated high-content screening to identify complex disease signatures in human fibroblasts. Nat Commun (2022) 13:1590 (opens in new window) L Schiff, B Migliori, B Johannesson et al.	2022
Hybridization-based in situ sequencing (HybISS) via direct RNA detection for identifying low abundance transcripts in mouse brain sections	Transcriptomics SPECTRA X Light Engine	Direct RNA targeted in situ sequencing for transcriptomic profiling in tissue. Sci Rep (2022) 12:7976 (opens in new window) H Lee, SM Salas, M Nilsson et al.	2022
Optogenetic induction of telomere coalescence	Optogenetics SPECTRA X Light Engine	Compartmentalization of telomeres through DNA- scaffolded phase separation. Dev Cell (2022) 57:277–290 (opens in new window) A Jack, Y Kim, CP Brangwynne et al.	2022
Quantitative immunofluorescence analysis of dystrophin expression in muscle biopsy tissue	Diagnostics SOLA Light Engine	Automated immunofluorescence analysis for sensitive and precise dystrophin quantification in muscle biopsies. Neuropathol Appl Neurobiol (2022) 48:e12785 (opens in new window) TA Vetter, S Nicolau, KM Flanagan et al.	2022
Imaging of labeled mRNA transcripts for validation of probe hybridization	Transcriptomics SPECTRA X Light Engine	Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis. Nat Commun (2022) 10:169 (opens in new window) T Vu, A Vallmitjana, W Zhao et al.	2022
One-photon photostimulation of fast-acting, high-conductance ChroME2.0 opsins	Optogenetics SPECTRA X Light Engine	High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks. Neuron (2022) 110:1–17 (opens in new window) S. Sridharan, MA Gajowa, H Adesnik et al.	2022
Evaluation of the photovoltaic effect in molybdenum disulfide (MoS₂) semiconductor flakes	Materials Science SOLA Light Engine	Mechanical stress modulation of resistance in MoS₂ junctions. Nano Lett (2022) 22:1047–1052 (opens in new window) P Chaudhary, H Lu, A Gruverman	2022
340/380 nm fura-2 imaging of dysregulated Ca²⁺ homeostasis in mouse fibroblasts	Live Cell Microscopy RETRA Light Engine	Ppt1-deficiency dysregulates lysosomal Ca²⁺ -homeostasis contributing to pathogenesis in a mouse model of CLN1 disease. J Inherit Metab Dis (2022) doi: 10.1002/jimd.12485 (opens in new window) A Mondal, AP Appu, AB Mukherjee et al.	2022
Super-resolution radial fluctuation (SRRF) microscopy of chromatin	Superresolution Microscopy SPECTRA Light Engine	Super-resolution radial fluctuations (SRRF) microscopy. Methods Mol Biol (2022) 2440:225–251 (opens in new window) J Salsman, G Dellaire	2022
Quantitative immunofluorescence analysis of tropomyosin expression in mesenchymal stromal/stem cells (MSCs)	Immunofluorescence Microscopy SPECTRA X Light Engine	Delineating the heterogeneity of matrix-directed differentiation toward soft and stiff tissue lineages via single-cell profiling. Proc Natl Acad Sci U S A (2021) 118:e2016322118 (opens in new window) S Brielle, D Bavli, A Buxboim et al.	2021
Image-based identification of CRISPR-induced phenotypes	High-Content Screening SPECTRA X Light Engine	High-content imaging-based pooled CRISPR screens in mammalian cells. J Cell Biol (2021) 220:e202008158 (opens in new window) X Yan, N Stuurman, RD Vale et al.	2021
Single-molecule RNA fluorescence in situ hybridization (smFISH) in Saccharomyces cerevisiae	Transcriptomics SPECTRA X Light Engine	Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae. STAR Protoc (2021) 2:100647 (opens in new window) HP Patel, I Brouwer, TL Lenstra	2021
Reflection interference contrast microscopy (RICM) of unlabeled microtubules.	Biophysics SOLA Light Engine	Self-repair protects microtubules from destruction by molecular motors. Nat Mater (2021) 20:883–891 (opens in new window) S Triclin, D Inoue, M Théry et al.	2021
Detection of streptavidin-phycoerythrin (SAPE) fluorescence linked to hybridization of exosomal microRNAs on hydrogel microparticles	Diagnostics SOLA Light Engine	Hydrogel-based hybridization chain reaction (HCR) for detection of urinary exosomal miRNAs as a diagnostic tool of prostate cancer. Biosens Bioelectron (2021) 192:113504 (opens in new window) J Kim, JS Shim, N Choi et al.	2021
DNA FISH chromatin tracing correlated with immunofluorescence detection of epigenetic markers in IMR-90 cells	Transcriptomics CELESTA Light Engine	TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations. Genome Biol (2021) 22:309 (opens in new window) Y Cheng, M Liu, S Wang et al.	2021
High-throughput neurite outgrowth analysis using organotypic explant cultures	High-Content Screening SPECTRA X Light Engine	ExplantAnalyzer: An advanced automated neurite outgrowth analysis evaluated by means of organotypic auditory neuron explant cultures. J Neurosci Methods (2021) 363:109341 (opens in new window) D Schmidbauer, S Fink, R Glueckert et al.	2021
Two-color immunofluorescence detection of SARS-CoV-2 spike protein localization on HEK293T cell membranes	Immunofluorescence Microscopy SOLA Light Engine	Rapid characterization of spike variants via mammalian cell surface display. Mol Cell (2021) 81:5099–5111 (opens in new window) K Javanmardi, CW Chou, IJ Finkelstein et al.	2021
Ultraviolet photoactivation of NP-caged isopropyl ß-D-1-thiogalactopyranoside (IPTG)	Optogenetics SOLA Light Engine	(Optochemical) control of synthetic microbial coculture interactions on a microcolony level. ACS Synth Biol (2021) 10:1308–1319 (opens in new window) A Burmeister, Q Akhtar, A Grünberger et al.	2021
Automated whole-slide scanning FISH analysis on formalin fixed, paraffin embedded (FFPE) specimens	Fluorescence in Situ Hybridization (FISH) SPECTRA Light Engine	Optimized workflow for digitalized FISH analysis in pathology. Diagn Pathol (2021) 16:42 (opens in new window) V Chea, V Pleiner, M Tinguely et al.	2021
Immunofluorescence detection of PI(4,5)P₂ and lipid phosphatase synaptojanin1 in cultured mouse astrocytes	Immunofluorescence Microscopy SPECTRA X Light Engine	Synaptojanin1 deficiency upregulates basal autophagosome formation in astrocytes. J Biol Chem (2021) 297:100873 (opens in new window) PY Pan, J Zhu, CF Dreyfus et al.	2021
Imaging of lipid droplets in Drosophila larvae	Confocal Microscopy CELESTA Light Engine	Arc1 and the microbiota together modulate growth and metabolic traits in Drosophila. Development (2021) 148:dev195222 (opens in new window) SA Keith, C Bishop, BM McCartney et al.	2021