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Dichroic Mirrors & Filters

In fluorescence microscopy, the filter set, consisting of excitation and emission bandpass filters and a dichroic beamsplitter, plays a critical role. It performs the essential functions of directing excitation light from the light source to the sample and then separating it on the basis of wavelength, from fluorescence emitted from the specimen. The filter set consists of excitation and emission bandpass filters and a dichroic beamsplitter. Optimized filter sets are critical because fluorescence emission from a microscopic specimen is many orders of magnitude (>10<sup>6</sup>) weaker than the excitation from the light source. Recognizing that optimized filter set specifications are critical for obtaining images with high signal:background contrast, Lumencor has developed uniquely high performance, multiband dichroic beamsplitters and multiband and single band emission filters for use with our SPECTRA, CELESTA, and ZIVA light engines®. Our multiband emitters and dichroics are optimized for compatibility with the electronically selectable excitation outputs of SPECTRA, CELESTA, and ZIVA light engines®. This enables fast multicolor imaging without the need for filter wheels or other positioning devices to execute filter interchanges. Single bandpass filters are offered for use in situations where fluorescence crosstalk (e.g. detection of FITC fluorescence derived from violet (DAPI) excitation) confounds identification of fluorescently labeled components of the specimen.

ProductPart NumberDescriptionFile

CELESTA/ZIVA VCGRnIR pentaband emitter

10-10857

Pentaband emission filter optimized for fluorescence microscopy using excitation from VCGRnIR output channels of CELESTA and ZIVA light engines. 25mm diameter in 3.5mm mounting ring. For detection of DAPI, FITC, TRITC, Cy5, Cy7 and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

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CELESTA/ZIVA VCGRnIR pentaband dichroic

10-10858

Pentaband dichroic optimized for fluorescence microscopy using excitation from VCGRnIR output channels of CELESTA and ZIVA light engines. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with pentaband emitter 10-10857.

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CELESTA/ZIVA BTY triple band emitter

10-10834

Triple band emission filter optimized for fluorescence microscopy using excitation from B and T output channels of CELESTA and ZIVA light engines. 25 mm diameter in 3.5 mm mounting ring For detection of CFP, YFP, mCherry and spectrally similar fluorophores in combination with triple band dichroic 10-10835.

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CELESTA/ZIVA BTY triple band dichroic

10-10835

Triple band dichroic optimized for fluorescence microscopy using excitation from B and T output channels of CELESTA and ZIVA light engines. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with triple band emitter 10-10834.

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Single band DAPI emitter, 440/37 nm

10-10871

Single band DAPI emitter, 440/37 nm, in mounting ring, 25.4 mm diameter. For detection of DAPI and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

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Single band FITC emitter, 510/31 nm

10-10872

Single band FITC emitter, 510/31 nm, in mounting ring, 25.4 mm diameter. For detection of FITC, GFP and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

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Single band TRITC emitter, 590/50 nm

10-10873

Single band TRITC emitter, 590/50 nm, in mounting ring, 25.4 mm diameter. For detection of TRITC, Cy3 and spectrally similar fluorophores in combination with pentaband dichroic 10-10858

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Single band Cy5 emitter, 691/64 nm

10-10874

Single band Cy5 emitter, 691/64 nm, in mounting ring, 25.4 mm diameter. For detection of Cy5 and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

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Single band Cy7 emitter, 823/100 nm

10-10875

Single band Cy7 emitter, 823/100 nm, in mounting ring, 25.4 mm diameter. For detection of Cy7 and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

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Single band emitter set DAPI/FITC/TRITC/Cy5/Cy7

10-10888

Set consists of single band emitters 10-10871, 10-10872, 10-10873, 10-10874 and 10-10875. For detection of DAPI, FITC, TRITC, Cy5 and Cy7 and spectrally similar fluorophores in combination with pentaband dichroic 10-10858.

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SPECTRA VCGRnIR pentaband emitter

10-10859

Pentaband emission filter optimized for fluorescence microscopy using excitation from VCGRnIR output channels of SPECTRA light engine. 25 mm diameter in 3.5 mm mounting ring. For detection of DAPI, FITC, TRITC, Cy5, Cy7 and spectrally similar fluorophores in combination with pentaband dichroic 10-10860.

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SPECTRA VCGRnIR pentaband dichroic

10-10860

Pentaband dichroic optimized for fluorescence microscopy using excitation from VCGRnIR output channels of SPECTRA light engine. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with pentaband emitter 10-10859.

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SPECTRA BTY triple band emitter

10-10836

Triple band emission filter optimized for fluorescence microscopy using excitation from BTY output channels of SPECTRA light engine. 25 mm diameter in 3.5 mm mounting ring. For detection of CFP, YFP, mCherry and spectrally similar fluorophores in combination with triple band dichroic 10-10837.

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SPECTRA BTY triple band dichroic

10-10837

Triple band dichroic optimized for fluorescence microscopy using excitation from BTY output channels SPECTRA light engines. Unmounted, 25 mm x 36 mm x 1 mm. For use in combination with triple band emitter 10-10836.

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All emission filters and dichroics have standard dimensions and are supplied in an unmounted form. Installation in filter cubes, filter wheels, or other instrumentation-specific mountings is required before use.