Application Bibliography
| Application | Area Product | Reference | Date |
|---|---|---|---|
Freestanding bilayer microscopy (FBM) of human mechanosensitive potassium channel (hTRAAK) in lipid bilayer membranes | Biophysics SPECTRA X Light Engine | Freestanding bilayer microscope for single-molecule imaging of membrane proteins. Sci Adv (2024) 10:eado4722 G Pérez-Mitta, Y Sezgin, R MacKinnon et al., | 2024 |
Label-free optical redox imaging (ORI) for monitoring human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) differentiation and maturation | High-Content Screening SOLA Light Engine | 2024 | |
Imaging of membrane potential in rat hippocampal neurons using the genetically encoded fluorescent voltage indicator ASAP3β | Live Cell Microscopy SOLA Light Engine | 2024 | |
High-throughput in situ sequencing interrogation of the expression of 104 cell-type marker genes at cellular resolution in the mouse cerebral cortex. | Transcriptomics CELESTA Light Engine | 2024 | |
Total internal reflection fluorescence (TIRF) microscopy of Ca2+ dynamics and granule fusions in insulin-secreting INS-1 cells | Live Cell Microscopy SPECTRA Light Engine | 2024 | |
Iterative indirect immunofluorescence imaging (4i) of retinoblastoma (Rb) phosphorylation in MCF-10A human mammary epithelial cells | Immunofluorescence Microscopy SPECTRA X Light Engine | An intermediate Rb–E2F activity state safeguards proliferation commitment. Nature (2024) 631:424–431 Y Konagaya, D Rosenthal, T Meyer et al. | 2024 |
Multiplexed immunofluorescence imaging on large (average area = 0.25 mm2) sections of healthy, regenerating, and embolized human liver tissues | Immunofluorescence Microscopy CELESTA Light Engine | Cell atlas of the regenerating human liver after portal vein embolization. Nat Commun (2024) 15:5827 A Brazovskaja, T Gomes, B Treutlein et al. | 2024 |
Cryo-fluorescence microscopy detection of MX04-labeled amyloid in postmortem Alzheimer’s disease brain tissue | Pathology SOLA Light Engine | CryoET of β-amyloid and tau within postmortem Alzheimer’s disease brain. Nature (2024) 631:913–919 MAG Gilbert, N Fatima, RAW Frank et al. | 2024 |
Optical control of protein dimerization via reversible photoisomerization of an azobenzene ligand | Optogenetics SPECTRA X Light Engine | 2024 | |
Time-lapse confocal spinning disk imaging of heterotypic human ovarian fibroblast + serous ovarian cancer spheroid invasion of mesothelial cell monolayers | Confocal Microscopy CELESTA Light Engine | 2024 | |
Imaging T-cell calcium signaling elicited by a biomimetic antigen-presenting system (bAPS) | Bioengineering SPECTRA X Light Engine | 2024 | |
Confocal immunofluorescence localization of Aurora-A kinase (AurkA) and its major regulator TPX2 in hTERT RPE-1 cells | Confocal Microscopy CELESTA Light Engine | 2024 | |
Time-lapse imaging of periplasmic mTurquoise2 and cytoplasmic mCherry in Escherichia coli | Microbiology SOLA Light Engine | SlyB encapsulates outer membrane proteins in stress-induced lipid nanodomains. Nature (2024) 626:617–625 A Janssens, VS Nguyen, H Remaut et al. | 2024 |
Immunofluorescence identification of metastatic prostate cancer cells based on epithelial cell adhesion molecule (EpCAM) expression | Diagnostics SOLA Light Engine | A magnetic microneedle to isolate single immunomagnetically labeled cells. Lab Chip (2024) 24:460–466 M Stevens, P Harder, LWMM Terstappen | 2024 |
Imaging laminar flow of peripheral blood mononuclear cells (PBMC) and neutrophils during microfluidic acoustophoresis | Microfluidics CELESTA Light Engine | Label‐free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing. Sci Rep (2024) 14:8748 J Alsved, MR Charan, P Augustsson et al. | 2024 |
Imaging fission and fusion of neuronal mitochondria expressing photoactivatable GFP (paGFP) and mScarlet | Live Cell Microscopy AURA Light Engine | 2024 | |
In situ sequencing (ISS) for single-cell spatial expression profiling of the spatiotemporal cellular dynamics underlying autoimmune encephalomyelitis (EAE) evolution. | Transcriptomics SPECTRA X Light Engine | Cellular architecture of evolving neuroinflammatory lesions P Kukanja, CM Langseth, G Castelo-Branco et al. | 2024 |
Real-time measurement of polymer–nucleotide substrate (avidite) binding to DNA polonies | Genomic Analysis RETRA Light Engine | Sequencing by avidity enables high accuracy with low reagent consumption. Nat Biotechnol (2024) 42:132–138 S Arslan, FJ Garcia, M Previte et al. | 2024 |
Immunofluorescence detection of long myosin light-chain kinase (MLCK) splice variants in FFPE ileal tissue microarrays from Crohn’s disease patients. | Pathology SOLA Light Engine, AURA Light Engine | 2024 | |
Imaging intracellular neuropeptide concentrations using a genetically encoded sensor comprising vasoactive intestinal peptide receptor VPAC1 coupled to circularly permuted GFP | Pathology SPECTRA Light Engine, SPECTRA X Light Engine | Multisensory gamma stimulation promotes glymphatic clearance of amyloid. Nature (2024) 627:149–156 MH Murdock, CY Yang, LH Tsai et al. | 2024 |
Vesicular glutamate transporter 1 (VGLUT1) mRNA FISH for identification and somal size measurement of human brain pyramidal neurons (PNs) | Fluorescence in Situ Hybridization (FISH) SPECTRA Light Engine | Scaling of smaller pyramidal neuron size and lower energy production in schizophrenia. Neurobiol Dis (2024) 191:106394 KE Schoonover, NE Miller, D Lewis et al. | 2024 |
Tracing interactions of polycomb group (PcG) proteins with chromatin by Optical Reconstruction of Chromatin Architecture (ORCA) | Transcriptomics CELESTA Light Engine | 2024 | |
Colocalization of antisense oligonucleotides and proteins using widefield 3D deconvolution microscopy | Immunofluorescence Microscopy AURA Light Engine | Profiling the interactome of oligonucleotide drugs by proximity biotinylation. Nat Chem Biol (2024) doi: 10.1038/s41589-023-01530-z A Hanswillemenke, DT Hofacker, T Stafforst | 2024 |
Structured illumination microscopy with 100 nm lateral and 300 nm axial resolution | Superresolution Microscopy CELESTA Light Engine | Easy access to super-resolution applications with DeepSIM. Microscopy Today (2024) 32, 10–15 C Brighi, L Clario, S Romanzi | 2024 |
Live-cell imaging for assessment of bacterial phenotype after antibiotic treatment | Microbiology SOLA Light Engine | 2024 |