Bibliography
Applications of Lumencor Light Engines
| Area | Lumencor Light Engine | Application | Reference | Authors | PMID | Year |
|---|---|---|---|---|---|---|
| Diagnostics | SPECTRA III | Immunofluorescence microscopy detection of EpCAM+/CD45-/DAPI+ circulating tumor cells (CTC) | An automatic platform based on nanostructured microfluidic chip for isolating and identification of circulating tumor cells. Micromachines (2021) 12:473 | HJ Jou, LY Chou, HT Hsu et al. | 33919456 | 2021 |
| Live Cell Microscopy | SOLA SE 365 | Timelapse imaging of intracellular Ca2+, mitochondrial membrane potential and cytosolic lactate | CDK2 limits the highly energetic secretory program of mature β cells by restricting PEP cycle-dependent K ATP channel closure. Cell Rep (2021) 34:108690 | SM Sdao, T Ho, MJ Merrins et al. | 33503433 | 2021 |
| Microfluidics | SOLA SE | Quantitation of Alexa Fluor 647-labeled DNA binding to GFP-tagged transcription factors | High-throughput affinity measurements of transcription factor and DNA mutations reveal affinity and specificity determinants. Cell Syst (2021) 12:112–127 | AK Aditham, CJ Markin, PM Fordyce et al. | 33340452 | 2021 |
| Immunofluorescence Microscopy | SPECTRA III | Immunofluorescence detection of SARS-CoV-2 viral nucleocapsid in Vero E6 cells | Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors. PLoS Pathog (2021) 17:e1009165 | M Mor, M Werbner, NT Freund et al. | 33571304 | 2021 |
| Transcriptomics | CELESTA | MERFISH imaging of chromatin organization and transcriptional activity | Genome-scale imaging of the 3D organization and transcriptional activity of chromatin. Cell (2020) 182:1641–1659 | JH Su, P Zheng, X Zhuang et al. | 32822575 | 2020 |
| Transcriptomics | SPECTRA X | Sequencing-by-hybridization for improved spatial detection of mRNA transcripts in human and mouse brain tissue | Hybridization-based in situ sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue. Nucleic Acids Res (2020) 48:e112 | D Gyllborg, CM Langseth, M Nilsson et al. | 32990747 | 2020 |
| Multimode Imaging | SPECTRA X | Ca2+ imaging of transgenic mice expressing GCaMP6f in cortical excitatory neurons | Simultaneous cortex-wide fluorescence Ca2+ imaging and whole-brain fMRI. Nat Methods (2020) 17:1262–1271 | EMR Lake, X Ge, RT Constable et al. | 33139894 | 2020 |
| Bioengineering | SPECTRA III | TIRF microscopy assay for detection of glycosphingolipid vesicle binding to surface-immobilized human norovirus VLPs | Histo-blood group antigens of glycosphingolipids predict susceptibility of human intestinal enteroids to norovirus infection. J Biol Chem (2020) 295:15974–15987 | I Rimkute, K Thorsteinsson, G Larson et al. | 32913124 | 2020 |
| Bioengineering | SOLA SE | Quantitative single-molecule analysis of membrane transporter activity | Monodisperse liposomes with femtoliter volume enable quantitative digital bioassays of membrane transporters and cell-free gene expression. ACS Nano (2020) 14:11700–11711 | N Soga, A Ota, H Noji et al. | 32864949 | 2020 |
| Bioengineering | SOLA SE 365 | Patterned ultraviolet photopolymerization of polyethyleneglycol diacrylate (PEGDA) | The role of valve stiffness in the insurgence of deep vein thrombosis. Commun Mater (2020) 1:65 | Z Schofield, HA Baksamawi, D Vigolo et al. | 32999999 | 2020 |
| High-Content Screening | SPECTRA X | Photoswitching of Dendra2 fluorescent protein for phenotypic annotation of single cells | High-throughput, microscope-based sorting to dissect cellular heterogeneity. Mol Syst Biol (2020) 16:e9442 | N Hasle, A Cooke, DM Fowler et al. | 32500953 | 2020 |
| Intravital Imaging | SOLA SE | Green>red photoswitching of kaede fluorescent protein for tracking of neutrophil migration | Hif-1α stabilisation is protective against infection in zebrafish comorbid models. FEBS J (2020) 287:3925–3943 | Y Schild, A Mohamed, PM Elks et al. | 32485057 | 2020 |
| Immunofluorescence Microscopy | SPECTRA X | Immunofluorescence detection of coronavirus receptors on Vero-E6 and BHK-R cells | Targeting polyamines inhibits coronavirus infection by reducing cellular attachment and entry. ACS Infect Dis (2021) 7:1423–1432 | MR Firpo, V Mastrodomenico, BC Mounce et al. | 32966040 | 2021 |
| Optogenetics | SOLA SE | Optogenetic manipulation of membrane curvature in live cells | Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS Synth Biol (2020) 9:893–901 | T Jones, A Liu, B Cui | 32212723 | 2020 |
| Immunofluorescence Microscopy | SPECTRA X | 4-color immunofluorescence microscopy of Drosophila S2 cells | Two-step mechanism for selective incorporation of lncRNA into a chromatin modifier. Nucleic Acids Res (2020) 48:7483–7501 | M Müller, T Schauer, PB Becker et al. | 32510132 | 2020 |
| Superresolution Microscopy | SPECTRA III | Super-resolution radial fluctuation (SRRF) microscopy of protein organization on the surface of nuclear lipid droplets (nLDs) | Lipid-associated PML structures assemble nuclear lipid droplets containing CCTα and Lipin1. Life Sci Alliance (2020) 3:e202000751 | J Lee, J Salsman, ND Ridgway et al. | 32461215 | 2020 |
| Genomic Analysis | SOLA SE FISH | Identification of fluorescent color-coded nanoliter droplets containing CRISPR-based nucleic acid detection reagents | Massively multiplexed nucleic acid detection with Cas13. Nature (2020) 582:277–282 | CM Ackerman, C Myhrvold, PC Sabeti et al. | 32349121 | 2020 |
| Materials Science | SOLA SM | Direct observation of graphene, molybdenum disulfide (MoS2), and hexagonal boron nitride (h-BN) particles at fluid−fluid interfaces using interference reflection microscopy (IRM) | Interference provides clarity: Direct observation of 2D materials at fluid-fluid interfaces. ACS Nano (2020) 14:777–790 | DM Goggin, H Zhang, JR Samaniuk et al. | 31820924 | 2020 |
| Materials Science | SOLA SM | Imaging water vapor condensation on hydrophobic–hydrophilic hybrid surfaces | High-throughput stamping of hybrid functional surfaces. Langmuir (2020) 36:5730–5744 | MJ Hoque, X Yan, N Miljkovic et al. | 32370513 | 2020 |
| Microfluidics | SPECTRA X | High speed (100 fps) imaging of HPTS (pH indicator) and calcein AM (viability indicator) in MDA-MB 231 human mammary gland/breast cancer cells | Microfluidic platform for the isolation of cancer-cell subpopulations based on single-cell glycolysis. Anal Chem (2020) 92:6949–6957 | C Zielke, CW Pan, P Abbyad et al. | 32297730 | 2020 |
| High-Content Screening | CELESTA | 4-color (DAPI, YFP, mCherry, Cy5) confocal imaging for identification of CRISPR knockout cell colonies with reduced stress granule abundance | Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Nat Methods (2020) 17:636–642 | EC Wheeler, AQ Vu, GW Yeo et al. | 32393832 | 2020 |
| Multimode Imaging | SPECTRA X | Illumination for single-wavelength (435 nm) spatial frequency domain imaging (SFDI), white light (390–630 nm) diffuse reflectance and endogeneous fluorescence (390 nm) imaging | Wide-field optical spectroscopy system integrating reflectance and spatial frequency domain imaging to measure attenuation-corrected intrinsic tissue fluorescence in radical prostatectomy specimens. Biomed Opt Express (2020) 17:2052–2072 | E Beaulieu, A Laurence, F Leblond et al. | 32341866 | 2020 |
| Bioengineering | SPECTRA X | 100 fps single particle tracking of 0.2 and 0.1 μm beads in hyaluronic acid hydrogels | Particle diffusion in extracellular hydrogels. Soft Matter (2020) 16:1366–1376 | F Burla F, T Sentjabrskaja, GH Koenderink et al. | 31939987 | 2020 |
| Confocal Microscopy | SPECTRA III | High-throughput 3D imaging of cell-induced matrix deformations using X-Light V2 spinning disk system | Mechanophenotyping of 3D multicellular clusters using displacement arrays of rendered tractions. Proc Natl Acad Sci U S A (2020) 117:5655–5663 | SE Leggett, M Patel, IY Wong et al. | 32123100 | 2020 |
| Fluorescence in Situ Hybridization (FISH) | CELESTA | Quantitation of NRF2 and MAFG expression in murine astrocytes using multiplexed error-robust FISH (MERFISH) | MAFG-driven astrocytes promote CNS inflammation. Nature (2020) 578:593–599 | MA Wheeler, IC Clark, FJ Quintana et al. | 32051591 | 2020 |
| High-Content Screening | SOLA SE FISH | Identification of CRISPR interference (CRISPRi) genotypes following phenotypic screening | Time-resolved imaging-based CRISPRi screening. Nat Methods (2020) 17:86–92 | D Camsund, MJ Lawson, J Elf et al. | 31740817 | 2020 |
| High-Content Screening | SPECTRA X | Long-term (12–24 hour) time-lapse imaging of GFP, YFP and RFP | Isolating live cells after high-throughput, long-term, time-lapse microscopy. Nat Methods (2020) 17:93–100 | S Luro, L Potvin-Trottier, J Paulsson et al. | 31768062 | 2020 |
| Immunofluorescence Microscopy | SPECTRA X | Quantitative immunofluorescence profiling of three-dimensional organoids formed by human mesenchymal stromal cells | Mapping the structure and biological functions within mesenchymal bodies using microfluidics. Sci Adv (2020) 6:eaaw7853 | S Sart, RFX Tomasi, CN Baroud et al. | 32181333 | 2020 |
| Microfluidics | SOLA SE FISH | Particle streak velocimetry for characterization of high-speed pulsatile fluid flow | Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction. Lab Chip (2020) 20:3744–3756 | T Qian, DA Gil, MC Skala et al. | 33048070 | 2020 |
| Immunofluorescence Microscopy | SOLA SM | Detection of IgG secretion from murine B cells compartmentalized in 0.05 nanoliter droplets | The Quantitative Assessment of the secreted IgG repertoire after recall to evaluate the quality of immunizations. J Immunol (2020) 205:1176–1184 | K Eyer, C Castrillon, J Baudry et al. | 32669311 | 2020 |
| Immunofluorescence Microscopy | SPECTRA | Immunfluorescence detection of cardiovascular remodeling markers in FFPE rat heart tissue sections | Statins reverse postpartum cardiovascular dysfunction in a rat model of preeclampsia. Hypertension (2020) 75:202–210 | K Kräker, JM O'Driscoll, N Haase et al. | 31786987 | 2020 |
| Spectral Imaging | SPECTRA | 12-color brightfield illumination for high-speed multiplexed biomarker detection | Brightfield multiplex immunohistochemistry with multispectral imaging. Lab Invest (2020) 100:1124–1136 | LE Morrison, MR Lefever, DR Bauer et al. | 32341516 | 2020 |
| Intravital Imaging | SPECTRA | Fluorescence endoscopic detection of colorectal neoplastic lesions | Topical application of Chlorin e6-PVP (Ce6-PVP) for improved endoscopic detection of neoplastic lesions in a murine colitis-associated cancer model. Sci Rep (2020) 10:13129 | I Ganzleben, M Hohmann, MJ Waldner et al. | 32753653 | 2020 |
| Intravital Imaging | SPECTRA X | Mesoscopic Ca2+ imaging in GCaMP6 transgenic mouse brain | Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits. Nat Methods (2020) 17:107–113 | D Barson, AS Hamodi, MJ Higley et al. | 31686040 | 2020 |
| Live Cell Microscopy | SPECTRA | Excitation of EuTTA (377/50 nm) and fluo-4 (485/20 nm) for measurement of intracellular temperature and Ca2+ in electrically stimulated hippocampal neurons | Single-cell temperature mapping with fluorescent thermometer nanosheets. J Gen Physiol (2020) 152:e201912469 | K Oyama, M Gotoh, N Fukuda et al. | 32421782 | 2020 |
| Live Cell Microscopy | SPECTRA X | Time-lapse imaging of GFP and mCherry for assessment of plasmolysis in live bacteria | Analysis of lipoprotein transport depletion in Vibrio cholerae using CRISPRi. Proc Natl Acad Sci USA (2019) 116:17013–17022 | F Caro, NM Place, JJ Mekalanos | 31371515 | 2019 |
| Live Cell Microscopy | SPECTRA X | High-speed (20 fps) widefield 3D microscopy of the endoplasmic reticulum | Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells. Sci Adv (2020) 6:eaay9572 | SD Carter, CM Hampton, Z Freyberg et al. | 32270040 | 2020 |
| Live Cell Microscopy | SPECTRA X | Time-lapse imaging of GFP and dsRed in S. pombe yeast cells | Size-dependent Increase in RNA polymerase II initiation rates mediates gene expression scaling with cell size. Curr Biol (2020) 30:1217–1230 | X Sun, A Bowman, S Marguerat et al. | 32059768 | 2020 |
| Optogenetics | SPECTRA III | Photostimulation (575/25 nm) of inhibitory halorhodopsin eNpHR3 in transgenic mouse brain slices | Light-induced silencing of neural activity in Rosa26 knock-in and BAc transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3. Sci Rep (2020) 10:3191. | I imayoshi, S Tabuchi, A Yamanaka et al. | 32081938 | 2020 |
| Optogenetics | SPECTRA X | Detection of optogenetically-induced RFP expression in S. cerevisiae yeast cells | Cell-in-the-loop pattern formation with optogenetically emulated cell-to-cell signaling. Nat Commun (2020) 11:1355 | ML Perkins, D Benzinger, M Khammash et al. | 32170129 | 2020 |
| Live Cell Microscopy | SPECTRA X | Traction force microscopy (TFM) of talin knockout cells on fibronectin coated polyacrylamide hydrogels | Relief of talin autoinhibition triggers a force-independent association with vinculin. J Cell Biol (2020) 219:e201903134 | P Atherton, F Lausecker, C Ballestrem et al. | 31816055 | 2020 |
| Transmitted Light Microscopy | PEKA | Phase contrast microscopy tracking of E. coli motility | Escherichia coli remodels the chemotaxis pathway for swarming. mBio (2019) 10:e00316–19 | JD Partridge, NTQ Nhu, RM Harshey et al. | 30890609 | 2019 |
| Transmitted Light Microscopy | PEKA | Phase contrast microscopy of Vibrio cholerae morphology | Vibrio cholerae adapts to sessile and motile lifestyles by cyclic di-GMP regulation of cell shape. Proc Natl Acad Sci U S A (2020) 117:29046–29054 | NL Fernandez, BY Hsueh, CM Waters et al. | 33139575 | 2020 |
| Materials Science | SOLA SM | Focal plane shift imaging of atmospheric water vapor condensation on superhydrophobic copper oxide nano-wires | Atmosphere-mediated superhydrophobicity of rationally designed micro/nanostructured surfaces. ACS Nano (2019) 13:4160–4173 | X Yan, Z Huang, N Miljkovic et al. | 30933473 | 2019 |
| Biophysics | SPECTRA X | Quantitation of low (<10) protein copy numbers in individual E. coli cells | Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy. Sci Rep (2019) 9:7902 | A Lepore, H Taylor, ME Karoui et al. | 31133640 | 2019 |
| Biophysics | AURA | Time-lapse imaging of fluorescence recovery after photobleaching (FRAP) in supported lipid bilayers (SLB) | Microtubule binding kinetics of membrane-bound kinesin-1 predicts high motor copy numbers on intracellular cargo. Proc Natl Acad Sci U S A (2019) 116:26564–26570 | R Jiang, S Vandal, WO Hancock et al. | 31822619 | 2019 |
| Biophysics | SPECTRA | Measurement of temperature-dependent europium (III) thenoyltrifluoroacetonate and rhodamine phalloidin fluorescence for characterization of infrared laser–induced local heating of actin filaments | Microscopic heat pulses activate cardiac thin filaments. J Gen Physiol (2019) 151:860–869 | S Ishii, K Oyama, S Ishiwata et al. | 31010810 | 2019 |
| Materials Science | SPECTRA X | Alternating red (650 nm) and blue (455 nm) irradiation of (PhZnRu)2+-functionalized photoelectrodes | Excitation energy-dependent photocurrent switching in a single-molecule photodiode. Proc Natl Acad Sci U S A (2019) 116:16198–16203 | B Shan, A Nayak, TJ Meyer et al. | 31366631 | 2019 |
| Bioengineering | SPECTRA X | Quantitative imaging of programmed YFP and mCherry expression in HEK-293T cells | A CRISPR/Cas9-based central processing unit to program complex logic computation in human cells. Proc Natl Acad Sci USA (2019) 116(15):7214–7219 | H Kim, D Bojara, M Fussenegger | 30923122 | 2019 |
| Bioengineering | SOLA SM | Visible light (409–650 nm) initiation of glycidyl methacrylate-dextran hydrogel photopolymerization | Comparison of visible and UVA phototoxicity in neural culture systems micropatterned with digital projection photolithography. J Biomed Mater Res A (2019) 107:134–144 | P Khoshakhlagh, DA Bowser, MJ Moore et al. | 30358101 | 2019 |
| Optogenetics | SPECTRA X | Evaluation of intensity–response characteristics of channelphodsin 2 evoked responses in rat dorsal root ganglion (DRG) neurons using 500 microsecond pulsed photostimulation at 2 to 78 mW | Optogenetic recruitment of spinal reflex pathways from large-diameter primary afferents in non-transgenic rats transduced with AAV9/Channelrhodopsin 2. J Physiol (2019) 597(19):5025–5040 | S Kubota, W Sidikejiang, K Seki et al. | 31397900 | 2019 |
| Microscopy Technique Development | SPECTRA X | Experimental validation of multifocal light field microscopy (MFLFM) | Snapshot multifocal light field microscopy. Opt Express (2020) 28:12108–12120 | K He, X Wang, O Cossairt et al. | 32403711 | 2020 |
| Superresolution Microscopy | SPECTRA | Super-resolution radial fluctuation (SRRF) microscopy of amyloid fibrils | An easy path for correlative electron and super-resolution light microscopy. Sci Rep (2019) 9:15526 | D Pinotsi , S Rodighiero, G Csucs et al. | 31664135 | 2019 |
| Superresolution Microscopy | SPECTRA X | 3-dimensional tracking of mCherry-labeled insulin granules in MIN6 cells | Nanoscale resolution 3D snapshot particle tracking by multifocal microscopy. Nano Lett (2019) 19(10):6781–6787 | X Wang, H Yi, NF Scherer et al. | 31490694 | 2019 |
| Genomic Analysis | AURA | Image-based discrimination of microbeam-irradiated and bystander IMR90 human lung fibroblasts for single-cell gene expression analysis | An integrated preprocessing approach for exploring single-cell gene expression in rare cells. Sci Rep (2019) 9:19758 | J Shang, D Welch, Q Lin et al. | 31875032 | 2019 |
| Genomic Analysis | SOLA SE FISH | In situ sequencing of RNA amplicons using dye-labeled terminators (G = 534/572 nm; T= 575/615 nm; A =635/680 nm; C= 661/732 nm) | Optical pooled screens in human cells. Cell (2019) 179(3):787–799 | D Feldman, A Singh, PC Blainey et al. | 31626775 | 2019 |
| High-Content Screening | SOLA SE | Single-cell identification and tracking in time-lapse image datasets | A cell segmentation/tracking tool based on machine learning. Methods Mol Biol (2019) 2040:399–422 | HS Deter, M Dies, J Buceta et al. | 31432490 | 2019 |
| High-Content Screening | SOLA SE | Image-based analysis of cell death induced by experimental cancer therapeutics | Identification of Smac mimetics as novel substrates for p-glycoprotein. Cancer Lett (2019) 440-441:126–134 | M Hugle, S Czaplinski, S Fulda et al. | 30312727 | 2019 |
| Immunofluorescence Microscopy | SPECTRA | 5-color (DAPI/FITC/AF555/AF594/Cy5) immunofluorescence detection in 4-micron FFPE human tissue sections | Optimisation of multiplex immunofluorescence for a non-spectral fluorescence scanning system. J Immunol Methods (2019) 472:25–34 | CC Anyaegbu, TF Lee-Pullen, MJ McCoy et al. | 31181211 | 2019 |
| Intravital Imaging | SPECTRA X | Excitation (395/25 nm) of ALA-derived protoporphyrin IX for intraoperative imaging | Evaluation of aminolevulinic acid-derived tumor fluorescence yields disparate results in murine and spontaneous large animal models of lung cancer. Sci Rep (2019) 9:7629 | JD Predina, J Runge, D Holt et al. | 31113971 | 2019 |
| Live Cell Microscopy | SPECTRA X | Time-lapse imaging of Voltron transmembrane voltage indicators in cultured rat hippocampal neurons | Bright and photostable chemigenetic indicators for extended in vivo voltage imaging. Science (2019) 365:699–704 | AS Abdelfattah, T Kawashima, ER Schreiter et al. | 31371562 | 2019 |
| Live Cell Microscopy | SPECTRA X | Mapping of CFP-, YFP- and mCherry-labeled protein fiducial markers on E. coli chromosomes | Direct imaging of the circular chromosome in a live bacterium. Nat Commun (2019) 10(1):2194 | F Wu, A Japaridze, C Dekker et al. | 31097704 | 2019 |
| Live Cell Microscopy | SPECTRA X | Time-lapse imaging of Ada DNA methyltransferase expression in E. coli | A quantitative model explains single-cell dynamics of the adaptive response in Escherichia coli. Biophys J (2019) 117(6):1156–1165 | S Uphoff | 31466698 | 2019 |
| Live Cell Microscopy | SPECTRA X | Determination of actin colocalization with the barbed end–binding proteins Mena and Vasp in murine hippocampal neurons | Processive flow by biased polymerization mediates the slow axonal transport of actin. J Cell Biol (2019) 218(1):112–124 | N Chakrabarty, P Dubey, S Roy et al. | 30401699 | 2019 |
| Live Cell Microscopy | SPECTRA X | Imaging of mitochondrial fusion in isolated mitochondria and mouse embryonic fibroblast (MEF) cells | A catalytic domain variant of mitofusin requiring a wildtype paralog for function uncouples mitochondrial outer-membrane tethering and fusion. J Biol Chem (2019) 294(20):8001–8014 | EA Engelhart, S Hoppins | 30936207 | 2019 |
| Live Cell Microscopy | SPECTRA X | 386/470 nm fluorescence excitation ratio imaging of pHluorin | Probing chemotaxis activity in Escherichia coli using fluorescent protein fusions. Sci Rep (2019) 9:3845 | C Roggo, N Carraro, JR van der Meer | 30846802 | 2019 |
| Live Cell Microscopy | SPECTRA X | Time-lapse imaging of transcription sites using MS2-labeled RNA probes | Intrinsic dynamics of a human gene reveal the basis of expression heterogeneity. Cell (2019) 176 (1-2):213–226 | J Rodriguez, G Ren, DR Larson et al. | 30554876 | 2019 |
| Live Cell Microscopy | SOLA SE | 20-hour time-lapse imaging of GFP expression in HuH7 human liver carcinoma cells | Correlation of mRNA delivery timing and protein expression in lipid-based transfection. Integr Biol (2019) 11:362–371 | A Reiser, D Woschée, J O Rädler et al. | 31850498 | 2019 |
| Environmental Science | SPECTRA X | Efficacy testing of novel algaecides by imaging phycocyanin (Ex: 555 nm) and chlorophyll (Ex: 440 nm) in cyanobacteria | N‐halamine derivatized nanoparticles with selective cyanocidal activity: potential for targeted elimination of harmful cyanobacterial blooms. Environ Sci Technol (2019) 53:9160–9170 | G Sadhasivam, C Gelber, OH Shapiro et al. | 31328506 | 2019 |
| Materials Science | SPECTRA X | Photoinitiation of topological changes in polymer–azobenzene complexes | Dynamically evolving surface patterns through light-triggered wrinkling erasure. Langmuir (2019) 35(4):875–881 | J Vapaavuori, TC Stimpson, JM Moran-Mirabal | 30532978 | 2019 |
| Materials Science | SOLA SM | Measurement of contact angles of liquid droplets with solid surfaces by focal plane shift imaging | In situ droplet microgoniometry using optical microscopy. ACS Nano (2019) 13(11):13343–13353 | H Cha, J Ma, N Miljkovic et al. | 31596565 | 2019 |
| Microfluidics | SOLA SE | Identification of microbial strains in nanoliter droplets by triplet fluorescent color coding | Massively parallel screening of synthetic microbial communities. Proc Natl Acad Sci U S A (2019) 116:12804–12809 | J Kehe, A Kulesa, PC Blainey et al. | 31186361 | 2019 |
| Microfluidics | RETRA | Continuous insulin sensing using measurements of FITC-insulin fluorescence anisotropy | Synchronized stimulation and continuous insulin sensing in a microfluidic human islet on a chip designed for scalable manufacturing. Lab Chip (2019) 19:2993–3010 | AL Glieberman, BD Pope, KK Parker et al. | 31464325 | 2019 |
| Microfluidics | SPECTRA X | Real-time visualization of single DNA molecules | A nanofluidic device for real-time visualization of DNA-protein interactions on the single DNA molecule level. Nanoscale (2019) 11(4):2071–2078 | R Öz, S Kk, F Westerlund | 30644945 | 2019 |
| Microfluidics | SPECTRA X | Quantitation of deGFP product of cell-free transcription/translation reactions in femtoliter droplets | On-demand production of femtoliter drops in microchannels and their use as biological reaction compartments. Anal Chem (2019) 91:3484–3491 | M Shojaeian, FX Lehr, S Hardt et al. | 30715859 | 2019 |
| Microscopy Technique Development | SPECTRA X | Ratiometric imaging of di-8-ANEPPS in HEK 293 cells during externally applied pulsed electrical polarization | Ratiometric widefield imaging with spectrally balanced detection. Biomed Opt Express (2019) 10:5385–5394 | S Yudovich, L Shani, S Weiss et al. | 31646053 | 2019 |
| Optogenetics | SPECTRA | Pulsed photostimulation of channelrhodopsin (2 ms, 20 Hz) in mouse hippocampal neurons | Population imaging of neural activity in awake behaving mice. Nature (2019) 574(7778):413–417 | KD Piatkevich, S Bensussen, X Han et al. | 31597963 | 2019 |
| Optogenetics | SPECTRA X | Photostimulation response characterization of engineered high-photocurrent channelrhodopsins | Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics. Nat Methods (2019) 16(11):1176–1184 | CN Bedbrook, KK Yang, FH Arnold et al. | 31611694 | 2019 |
| Optogenetics | SPECTRA X | Generation of repetitive action potentials by 2-ms pulsed photostimulation of channelrhodopsin (470 nm; 4.9 mW/mm2) | Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of- magnitude reductions in calcium and proton currents. J Biol Chem (2019) 294(11):3806–3821 | YK Cho, D Park, ES Boyden et al. | 30610117 | 2019 |
| Photochemistry | SPECTRA | Control of photocurrent direction in a dye-sensitized photoelectrode via alternating red (650 nm) and blue (455 nm) light irradiation | Proc Natl Acad Sci U S A (2019) 116:16198–16203 | B Shan, A Nayak, TJ Meyer et al. | 31366631 | 2019 |
| Bioengineering | SPECTRA X | Optogenetic manipulation of cyclic dimeric guanosine monophosphate (c-di-GMP) levels in Pseudomonas aeruginosa biofilms | Bioprinting living biofilms through optogenetic manipulation. ACS Synth Biol (2018) 7(5):1195–1200 | Y Huang, A Xia, F Jin et al. | 29664610 | 2018 |
| Confocal Microscopy | SPECTRA X | Time-lapse imaging of actin and myosin-2 at single-filament level using X-Light spinning disk system | Polarity sorting drives remodeling of actin-myosin networks. J Cell Sci (2018) 132(4):jcs219717 | V Wollrab, JM Belmonte, GH Koenderink et al. | 30404824 | 2018 |
| Fluorescence in Situ Hybridization (FISH) | SOLA SE | Real-time monitoring of in situ hybridization rates | Real-time monitoring of fluorescence in situ hybridization kinetics. Anal Chem (2018) 90(19):11470–11477 | N Ostromohov, D Huber, GV Kaigala et al. | 30125088 | 2018 |
| Fluorescence in Situ Hybridization (FISH) | SPECTRA X | 5-color single-molecule FISH (smFISH) imaging of RNA transcripts | Spatial organization of the somatosensory cortex revealed by osmFISH. Nat Methods (2018) 15(11):932–935 | S Codeluppi, LE Borm, S Linnarsson et al. | 30377364 | 2018 |
| Genomic Analysis | SOLA SM | Fluorescence imaging of hydrogel microposts in a plastic PCR chip | Hydrogel micropost-based qPCR for multiplex detection of miRNAs associated with Alzheimer's disease. Biosens Bioelectron (2018) 101:235–244 | W Choi, SY Yeom, N Choi et al. | 29096361 | 2018 |
| High-Content Screening | SPECTRA | Automated identification of f-actin enriched invadosomes in NIH-3T3-Src cells | Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation. Nat Commun (2018) 9:2031 | Z Ezzoukhry, E Henriet, F Saltel et al. | 29795195 | 2018 |
| High-Content Screening | AURA | Image-based analysis of cytotoxicity and cytoskeletal integrity in iPSC-derived skeletal myoblasts. | High-content assay multiplexing for muscle toxicity screening in human-induced pluripotent stem cell-derived skeletal myoblasts. Assay Drug Dev Technol (2018) 16(6):333–342 | WD Klaren, I Rusyn | 30070899 | 2018 |
| Diagnostics | SOLA SE | Enhanced detection of CD45+ leukocytes immunolabled with allophycocyanin (APC) | Classification of cells in CTC-enriched samples by advanced image analysis. Cancers (2018) 10:E377 | S de Wit, LL Zeune, LW Terstappen et al. | 30308977 | 2018 |
| Immunofluorescence Microscopy | SPECTRA X | MicroRNA FISH and cytokeratin immunofluorescence detection in FFPE tissue sections by confocal slide scanning microscopy | miR-21 expression analysis in budding colon cancer cells by confocal slide scanning microscopy. Clin Exp Metastasis (2018) 35:819–830 | KN Knudsen, J Lindebjerg, BS Nielsen et al. | 30361805 | 2018 |
| Immunofluorescence Microscopy | SPECTRA X | Analysis of of intra-Golgi decorin and glycosaminoglycan levels in gerodermia osteodysplastica (GO) fibroblasts | Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica. PLoS Genet (2018) 14(3):e1007242 | WL Chan, M Steiner, U Kornak et al. | 29561836 | 2018 |
| Intravital Imaging | SPECTRA | In situ 400 nm photopolymerization of a sustained release formulation of paclitaxel | Post-resection treatment of glioblastoma with an injectable nanomedicine-loaded photopolymerizable hydrogel induces long-term survival. Int J Pharm (2018) 548(1):522–529 | M Zhao, F Danhier, V Préat et al. | 30017818 | 2018 |
| Intravital Imaging | SPECTRA X | Ca2+ imaging in salt-sensing ASE neurons of C. elegans | Loss of CaMKI function disrupts salt aversive learning in C. elegans J Neurosci (2018) 38:6114–6129 | JP Lim, H Fehlauer, MB Goodman et al. | 29875264 | 2018 |
| Live Cell Microscopy | SPECTRA X | Photoactivation (395 nm, 100 ms@0.1Hz, 16 mW/mm2) of Ca2+-dependent fluorescent protein CaMPARI2 | Improved methods for marking active neuron populations. Nat Commun (2018) 9:4440 | B Moeyaert, G Holt, ER Schreiter et al. | 30361563 | 2018 |
| Live Cell Microscopy | SOLA SE | Imaging of Vipp1–GFP (ex = 470 nm) and thylakoid membrane autofluorescence (ex = 640 nm) in live cyanobacterial cells | Dynamical localization of a thylakoid membrane binding protein is required for acquisition of photosynthetic competency. Mol Microbiol (2018) 108(1):16–31 | A Gutu, F Chang, EK O'Shea | 29357135 | 2018 |
| Live Cell Microscopy | SOLA SE | Imaging of ROS sensor roGFP2 in MCF10A cells | Inhibition of epithelial cell migration and Src/FAK signaling by SIRT3. Proc Natl Acad Sci U S A (2018) 115(27):7057–7062 | JJ Lee , RAH van de Ven, MC Haigis et al. | 29915029 | 2018 |
| Live Cell Microscopy | SOLA SE | Time-lapse imaging of EGFP-NFM and EB3-mCherry in rat cortical neurons | Kymograph analysis with high temporal resolution reveals new features of neurofilament transport kinetics. Cytoskeleton (2018) 75(1):22–41 | JD Fenn, CM Johnson, A Brown et al. | 28926211 | 2018 |
| Live Cell Microscopy | SPECTRA X | Calcium imaging of GPCR activation by peptide agonist ExONatide using fluo-8 (ex = 470/24 nm) | Conditional and reversible activation of class A and B G protein-coupled receptors using tethered pharmacology. ACS Cent Sci (2018) 4:166–179 | T Podewin, J Ast, DJ Hudson et al. | 29532016 | 2018 |
| Live Cell Microscopy | MIRA | Visualizing intercellular coupling via propidium iodide dye transfer in murine cerebral endothelial tubes | Electrical dynamics of isolated cerebral and skeletal muscle endothelial tubes: Differential roles of G-protein-coupled receptors and K+ channels. Pharmacol Res Perspect (2018) 6(2):e00391 | MA Hakim, JN Buchholz, EJ Behringer | 29636977 | 2018 |
| Microfluidics | SOLA SE | Simultaneous excitation of four fluorogenic protease substrates for detection of multiplexed matrix metalloproteinase (MMP) activity | Single-cell assays using integrated continuous-flow microfluidics. Methods Enzymol (2019) 628:59–94 | EX Ng, MN Hsu, CH Chen et al. | 31668236 | 2019 |
| Microfluidics | SOLA SE | Time-lapse imaging of microfluidic fractionation of bacteria based on chain length | Separation of pathogenic bacteria by chain length. Anal Chim Acta (2018) 1000:223–231 | JP Beech, BD Ho, JO Tegenfeldt et al. | 29289314 | 2018 |
| Microscopy Technique Development | SOLA SE | Interference reflection microscopy (IRM) of unlabeled microtubules | Label-free high-speed wide-field imaging of single microtubules using interference reflection microscopy. J Microsc (2018) 272(1):60–66 | M Mahamdeh, S Simmert, J Howard et al. | 30044498 | 2018 |
| Optogenetics | SPECTRA X | Light-controlled inhibition of AMPA receptor endocytosis mediated by PhotonSABER (photon-sensitive ASR-based endocytosis regulator) | Optogenetic control of synaptic AMPA receptor endocytosis reveals roles of LTD in motor learning. Neuron (2018) 99:985–998 | W Kakegawa, A Katoh, S Matsuda et al. | 30122381 | 2018 |
| Optogenetics | SPECTRA X | Optogenetic suppression of G-protein coupled receptor (GPCR) signaling (475 nm; 7.37 mW/mm2) | Optogenetic inhibition of Gαq protein signaling reduces calcium oscillation stochasticity. ACS Synth Biol (2018) 7(6):1488–1495 | P Hannanta-Anan, BY Chow | 29792810 | 2018 |
| Optogenetics | SOLA SE | Photoactivation of proteorhodopsin (510–560 nm) | Painting with light-powered bacteria. Nat Commun (2018) 9(1):768 | J Arlt, VA Martinez, Poon WCK et al. | 29472614 | 2018 |
| Photochemistry | SPECTRA | Optical control of ATP synthesis in photosynthetic artificial organelles | Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular system. Nat Biotechnol (2018) 36(6):530–535 | KY Lee, SJ Park, K Shin et al. | 29806849 | 2018 |
| Superresolution Microscopy | SPECTRA X | Structured illumination microscopy (SIM) visualization of actin and mitochondria in BPAE cells | Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction. GigaScience (2018) 8:1–12 | J Pospíšil, T Lukeš, GM Hagen et al. | 30351383 | 2018 |
| Bioengineering | SOLA SE | Identification of p-coumaric acid producing yeast based on RFP expression. | Development of a bacterial biosensor for rapid screening of yeast p-coumaric acid production. ACS Synth Biol (2017) 6(10):1860–1869 | S Siedler, NK Khatri, HN Joensson et al. | 28532147 | 2017 |
| Bioengineering | SOLA SE | Time lapse imaging of promoter activation and plasmid copy number in transformed E. coli. | Synchronized DNA cycling across a bacterial population. Nat Genet (2017) 49(8):1282–1285 | L Baumgart, W Mather, J Hasty | 28692067 | 2017 |
| Bioengineering | SOLA SE | Excitation of CellTracker Blue CMAC and GFP for image-based determination of plasmid transfection versus cell number | Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. Gene (2017) 626:200–208 | JS Osorio, M Bionaz | 28501631 | 2017 |
| Confocal Microscopy | SPECTRA X | Excitation of mCherry for live cell confocal imaging using X-Light spinning disk system | In vivo imaging of DNA double-strand break induced telomere mobility during alternative lengthening of telomeres. Methods (2017) 114:54–59 | NW Cho, MA Lampson, RA Greenberg | 27491801 | 2017 |
| Genomic Analysis | SPECTRA X | Excitation of EvaGreen dye for high resolution melting (dHRM) analysis of bacterial pathogen DNA sequences | Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling. Sci Rep. (2017) 7:42326 | DO Velez, H Mack, SI Fraley et al. | 28176860 | 2017 |
| High-Content Screening | SOLA SE | Imaging of RAW 264.7 macrophage infection by DsRed-expressing Mycobacterium tuberculosis | A high content microscopy assay to determine drug activity against intracellular Mycobacterium tuberculosis. Methods (2017) 127:3–11 | AJ Manning, Y Ovechkina, T Parish et al. | 28366666 | 2017 |
| Immunofluorescence Microscopy | SPECTRA X | 3-color expansion microscopy (ExM) imaging of DAPI, vimentin and keratin in human tissue specimens | Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy. Nat Biotechnol (2017) 35(8):757–764 | Y Zhao, O Bucur, ES Boyden et al. | 28714966 | 2017 |
| Spectral Imaging | SPECTRA | In-vivo multispectral imaging for detection of stomach carcinoma | In-vivo multispectral video endoscopy towards in-vivo hyperspectral video endoscopy. J Biophotonics (2017) 10(4):553–564 | M Hohmann, R Kanawade, H Albrecht et al. | 27403639 | 2017 |
| Intravital Imaging | SPECTRA X | Time-lapse fluorescence ratio imaging of HyPer-transgenic zebrafish using blue (438/57 nm) and cyan (475/28 nm) excitation | Image-based measurement of H2O2 reaction-diffusion in wounded zebrafish larvae. Biophys J (2017) 112(9):2011–2018 | M Jelcic, B Enyedi, P Niethammer et al. | 28494970 | 2017 |
| Live Cell Microscopy | SPECTRA X | Time-lapse imaging of microtubule dynamics in LLC-PK1 porcine epithelial cells stably expressing EGFP–α-tubulin or EB1–EGFP | Mechanisms of kinetic stabilization by the drugs paclitaxel and vinblastine. Mol Biol Cell (2017) 28(9):1238–1257 | BT Castle, S McCubbin, DJ Odde et al. | 28298489 | 2017 |
| Live Cell Microscopy | SPECTRA X | Fluorescence ratio imaging of H2O2 sensor HyPer with alternating 100 ms excitation pulses at 475/28 nm and 438/24 nm | Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure. Proc Natl Acad Sci U S A (2017) 114(34):9200–9205 | O Rodrigues, G Reshetnyak, L Verdoucq et al. | 28784763 | 2017 |
| Live Cell Microscopy | SPECTRA X | Excitation of CFP (434/17 nm), GFP (488/10 nm) and mCherry (560/25 nm) | Correlative live cell and super resolution imaging of autophagosome formation. Methods Enzymol (2017) 587:1–20 | SA Walker, E Karanasios, NT Ktistakis | 28253951 | 2017 |
| Live Cell Microscopy | SPECTRA X | Fluorescence microscopy of iRFP in cultured neurons and fixed brain slices | Near-infrared fluorescent proteins engineered from bacterial phytochromes in neuroimaging. Biophys J (2017) 113(10):2299–2309 | KD Piatkevich, Suk HJ, VV Verkhusha et al. | 29017728 | 2017 |
| Materials Science | SPECTRA X | Measurement of photoluminescence quantum yields of hybrid perovskite films | Correlation between photoluminescence and carrier transport and a simple in situ passivation method for high-bandgap hybrid perovskites. J Phys Chem Lett (2017) 8(14):3289–3298 | RJ Stoddard, FT Eickemeyer, HW Hillhouse et al. | 28636388 | 2017 |
| Materials Science | SOLA SE | Optical pumping of plasmonic laser (spaser) prototypes | A customizable class of colloidal-quantum-dot spasers and plasmonic amplifiers. Sci Adv (2017) 3(9):e1700688 | SJP Kress, J Cui, DJ Norris et al. | 28948219 | 2017 |
| Microfluidics | SOLA SE | Photobleaching of Texas Red–DHPE and Alexa Fluor 488 streptavidin for FRAP analysis of molecular diffusion | Dual-functional lipid coating for the nanopillar-based capture of circulating tumor cells with high purity and efficiency. Langmuir (2017) 33(4):1097–1104 | HY Lou, W Zhao, B Cui et al. | 28059522 | 2017 |
| Microscopy Technique Development | SPECTRA X | Acquisition of whole slide images (WSIs) for validation of BaSiC shading correction method | A BaSiC tool for background and shading correction of optical microscopy images. Nat Commun (2017) 8:14836 | T Peng, K Thorn, N Navab et al. | 28594001 | 2017 |
| Microscopy Technique Development | SOLA SE | Evaluating optical aberrations in single cell images using gold nanostructures as a reference framework | Single-cell optical distortion correction and label-free 3D cell shape reconstruction on lattices of nanostructures. Nano Lett (2017) 17(12):8018–8023 | J Stephan, F Keber, P Paulitschke et al. | 29199833 | 2017 |
| Multimode Imaging | SPECTRA X | Fluorescence excitation of paxilin-GFP and Alexa Fluor 594 immunolabeled insulin for correlative light and electron microscopy | Automated sub-5 nm image registration in integrated correlative fluorescence and electron microscopy using cathodoluminescence pointers. Sci Rep (2017) 7:43621 | MT Haring, N Liv, JP Hoogenboom et al. | 28252673 | 2017 |
| Multimode Imaging | SOLA SE | Cryogenic fluorescence microscopy of high-pressure frozen cells on sapphire disks | triCLEM: Combining high-precision, room temperature CLEM with cryo-fluorescence microscopy to identify very rare events. Methods Cell Biol (2017) 140:303–320 | NR Ader, W Kukulski | 28528638 | 2017 |
| Optogenetics | SPECTRA X | Imaging mitochondrial Ca2+ responses to optogenetic modulation of mitochondrial membrane potential | Optogenetic control of mitochondrial metabolism and Ca2+ signaling by mitochondria-targeted opsins. Proc Natl Acad Sci U S A (2017) 114(26):E5167–E5176 | T Tkatch, E Greotti, I Sekler et al. | 28611221 | 2017 |
| Optogenetics | SPECTRA X | Photoinitiation of halorhodopsin-mediated inhibition of murine cholecystokinin (CCK) neurons | Initiation of Behavioral Response to Antidepressants by cholecystokinin neurons of the dentate gyrus. Neuron (2017) 95(3):564–576 | L Medrihan, Y Sagi, P Greengard et al. | 28735749 | 2017 |
| Optogenetics | SPECTRA X | Activation (475/28 nm; 8 mW/mm2) and deactivation (632/22 nm; 5 mW/mm2) of step-function opsin (SSFO) and the step-function inhibitory opsin SwiChR++ | Modulation of prefrontal cortex excitation/inhibition balance rescues social behavior in CNTNAP2-deficient mice. Sci Transl Med (2017) 9:401 | A Selimbeyoglu, CK Kim, K Deisseroth et al. | 28768803 | 2017 |
| Optogenetics | RETRA | Optogenetic activation (475 nm) and deactivation (575 nm) of stablized step-function opsin (SSFO) in mouse claustral neurons | Organization of the claustrum-to-entorhinal cortical connection in mice. J Neurosci (2017) 37(2):269–280 | T Kitanishi, N Matsuo | 28077707 | 2017 |
| Bioengineering | SOLA SE | Excitation of autofluorescence for time-lapse imaging of cyanobacteria and algae. | A microfluidic platform for long-term monitoring of algae in a dynamic environment. ACS Synth Biol (2016) 5(1):8–14 | CS Luke, J. Selimkhanov, J Hasty et al. | 26332284 | 2016 |
| Confocal Microscopy | SPECTRA X | Imaging of DAPI, Alexa Fluor 488 and Cy3 immunofluorescence using X-Light spinning disk system | Differentiation of control and ALS mutant human iPSCs into functional skeletal muscle cells, a tool for the study of neuromuscular diseases. Stem Cell Res (2016) 17(1):140–147 | J Lenzi, F Pagani, A Rosa et al. | 27318155 | 2016 |
| Fluorescence in Situ Hybridization (FISH) | SOLA SE | Excitation of Hoechst 33342 nuclear stain and PlatinumBright 550-labeled centromere probes | Micro fluorescence in situ hybridization (μFISH) for spatially multiplexed analysis of a cell monolayer. Biomed Microdevices (2016) 18(2):40 | D Huber, J Autebert, GV Kaigala | 27138995 | 2016 |
| Fluorescence in Situ Hybridization (FISH) | SPECTRA X | Excitation of Cy3 labeled RNA probes for single molecule FISH detection | Single-gene dual-color reporter cell line to analyze RNA synthesis in vivo. Methods (2016) 103:77–85 | M Palangat, DR Larson | 27068658 | 2016 |
| Genomic Analysis | SPECTRA X | Real-time detection of multiple displacement DNA amplification (MDA) reactions in hydrogels | Virtual microfluidics for digital quantification and single-cell sequencing. Nat Methods (2016) 13(9):759–762 | L Xu, IL Brito, PC Blainey et al. | 27479330 | 2016 |
| High-Content Screening | SPECTRA X | Imaging of binucleated cells (mitotic index) for assessment of cell cycle progression | Cdk1 activity acts as a quantitative platform for coordinating cell cycle progression with periodic transcription. Nat Commun (2016) 7:11161 | G Banyai, F Baïdi, Z Szilagyi et al. | 27045731 | 2016 |
| High-Content Screening | SOLA SE | Bacterial cell identification by computationally enhanced phase contrast microscopy | Oufti: an integrated software package for high-accuracy, high-throughput quantitative microscopy analysis. Mol Microbiol (2016) 99(4):767–777 | A Paintdakhi, B Parry, C Jacobs-Wagner et al. | 26538279 | 2016 |
| High-Content Screening | SOLA SE | 5 channel (DAPI/FITC/Cy3/Texas Red/Cy5) excitation for cellular morphology profiling | Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. Nat Protoc (2016) 11(9):1757–1774 | MA Bray, S Singh, AE Carpenter et al. | 27560178 | 2016 |
| High-Content Screening | SPECTRA X | Excitation of GFP, YFP and mCherry for automated bacterial morphology classification | MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis. Nat Microbiol (2016) 1(7):16077 | A Ducret, EM Quardokus, YV Brun | 27572972 | 2016 |
| Intravital Imaging | SOLA SM | Large-scale Ca2+ imaging in GCaMP6 transgenic mouse brain | Long-term optical access to an estimated one million neurons in the live mouse cortex. Cell Rep (2016) 17(12):3385–3394 | TH Kim, Y Zhang, MJ Schnitzer et al. | 28009304 | 2016 |
| Intravital Imaging | SPECTRA X | Bright-field and autofluorescence imaging of C. elegans every 3 hours for up to 16 days | Extended twilight among isogenic C. elegans causes a disproportionate scaling between lifespan and health. Cell Syst (2016) 3(4):333–345 | WB Zhang, DB Sinha, Z Pincus et al. | 27720632 | 2016 |
| Live Cell Microscopy | SPECTRA X | Excitation of green fluorescent (GCaMP6m) and red fluorescent (R-CaMP2) genetically encoded calcium indicators in rat hippocampal neurons | Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain. Nat Methods (2016) 13(4):325–328 | CK Kim, SJ Yang, K Deisseroth et al. | 26878381 | 2016 |
| Live Cell Microscopy | SOLA SE 365 | Imaging of FRET biosensors for pyruvate kinase activity and ATP/ADP and endogenous NADPH and flavins | Phase analysis of metabolic oscillations and membrane potential in pancreatic islet β-cells. Biophys J (2016) 110(3):691–699 | MJ Merrins, C Poudel, LS Satin et al. | 26840733 | 2016 |
| Live Cell Microscopy | SPECTRA X | Imaging origins of replication loci (oriC) in E. coli expressing LacI-tagGFP | CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res (2016) 44(8):3801–3810 | J Wiktor, C Lesterlin, C Dekker et al. | 27036863 | 2016 |
| Live Cell Microscopy | SPECTRA | Excitation of GFP and mCherry in cultured hippocampal neurons | Axonal actin in action: Imaging actin dynamics in neurons. Methods Cell Biol (2016) 131:91–106 | K Ladt, A Ganguly, S Roy | 26794509 | 2016 |
| Live Cell Microscopy | SOLA SE | Imaging GFP-labeled endogenous β-actin mRNA in cultured neurons | Imaging single mRNA dynamics in live neurons and brains. Methods Enzymol (2016) 572:51–64 | HC Moon, HY Park | 27241749 | 2016 |
| Materials Science | SOLA SM | Focal plane shifted time-lapse imaging of water droplets on superhydrophobic surfaces | Focal plane shift imaging for the analysis of dynamic wetting processes. ACS Nano (2016) 10(9):8223–8232 | H Cha, JM Chun, N Miljkovic et al. | 27447844 | 2016 |
| Microfluidics | SOLA SE | Microfluidics-assisted cell screening (MACS) of E. coli | Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells. Nat Commun (2016) 7:11641 | B Okumus, D Landgraf, J Paulsson et al. | 27189321 | 2016 |
| Microfluidics | SOLA SE | Imaging of captured cells in proof-of-concept device for microfluidic diagnostics of large, dilute samples | Ultra-high-throughput sample preparation system for lymphocyte immunophenotyping point-of-care diagnostics. J Lab Autom (2016) 21:706–712 | DI Walsh III, SK Murthy, A Russom | 26920576 | 2016 |
| Microfluidics | SOLA SE | Photoactivation of MNI-caged glutamate | One-photon and two-photon stimulation of neurons in a microfluidic culture system. Lab Chip (2016) 16(9):1684–1690 | JM Jang, J Lee W Jung et al. | 27053163 | 2016 |
| Microfluidics | SOLA SE | Imaging micropattered goat anti-IgG deposition on IgG-coated polystyrene surfaces | Convection-enhanced biopatterning with recirculation of hydrodynamically confined nanoliter volumes of reagents. Anal Chem (2016) 88(6):3235–3242 | J Autebert, JF Cors, G V Kaigala et al. | 26837532 | 2016 |
| Microscopy Technique Development | SPECTRA X | Validation of expansion microscopy (ExM) on cells and tissues with multicolor immunofluorescence and fluorescent protein labeling | Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol (2016) 34(9):987–992 | PW Tillberg, F Chen, ES Boyden et al. | 27376584 | 2016 |
| Optogenetics | SPECTRA | Stimulation (ChR2) and inhibition (Arch-T) of transmembrane potassium flux in developing Xenopus embryos | Bioelectric signalling via potassium channels: a mechanism for craniofacial dysmorphogenesis in KCNJ2-associated Andersen-Tawil Syndrome. J Physiol (2016) 594(12):3245–3270 | DS Adams, SG Uzel, M Levin et al. | 26864374 | 2016 |
| Optogenetics | SPECTRA X | (1) Activation of photosensitive G protein-coupled receptor melanopsin (470 nm). (2) Excitation of X-rhod-1 Ca2+ indicator (570 nm) | Optogenetic control of calcium oscillation waveform defines NFAT as an Integrator of calcium load. Cell Syst (2016) 2(4):283–288 | P Hannanta-Anan, BY Chow | 27135540 | 2016 |
| Photochemistry | SPECTRA | 75 mW/cm2 illumination of zinc porphyrin MPZnP photosensitizer at 445 nm | Growth and post-deposition treatments of SrTiO3 films for dye-sensitized photoelectrosynthesis cell applications. ACS Appl Mater Interfaces (2016) 8(19):12282–12290 | RW Call, L Alibabaei, R Lopez et al. | 27128813 | 2016 |
| Bioengineering | SOLA SE | Imaging of GFP and RFP expression for monitoring gene deletion in E. coli | Targeted large-scale deletion of bacterial genomes using CRISPR-nickases. ACS Synth Biol (2015) 4(11):1217–1225 | K Standage-Beier, Q Zhang, X Wang | 26451892 | 2015 |
| Confocal Microscopy | SPECTRA X | Excitation of fluorescent protein redox sensors for confocal imaging using Yokogawa CSU-X1 spinning disk system | Redox sensor proteins for highly sensitive direct imaging of intracellular redox state. Biochem Biophys Res Commun (2015) 457(3):242–248 | K Sugiura, T Nagai, T Hisabori et al. | 25592971 | 2015 |
| Fluorescence in Situ Hybridization (FISH) | SOLA SE | Excitation of JF549 and JF646 fluorophores on dCas9/sgRNA hybridization probes | CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc Natl Acad Sci U S A (2015) 112(38):11870–11875 | W Deng, X Shi, RH Singer et al. | 26324940 | 2015 |
| FRET | SPECTRA X | Excitation of EKAR2G CFP/YFP FRET biosensor for imaging of ERK activation | Frequency modulation of ERK activation dynamics rewires cell fate. Mol Syst Biol (2015) 11(11):838 | H Ryu, M Chung, O Pertz et al. | 26613961 | 2015 |
| FRET | SOLA SE | Excitation of donor (mTurquoise) and acceptor (NeonGreen) fluorescent proteins in a vinculin activation FRET biosensor. | Molecular mechanism of vinculin activation and nano-scale spatial organization in focal adhesions. Nat Cell Biol (2015) 17(7):880–892 | LB Case, MA Baird, CM Waterman et al | 26053221 | 2015 |
| High-Content Screening | SOLA SE | 5 channel fluorescence excitation for image-based assessment of RNAi-induced phenotypes. | Morphological profiles of RNAi-induced gene knockdown are highly reproducible but dominated by seed effects. PLoS ONE (2015) 10(7):e013137. | S Singh, X Wu, AE Carpenter et al. | 26197079 | 2015 |
| High-Content Screening | SPECTRA X | Excitation of Hoechst 33342 (379 nm) and deglycosylation-dependent Venus YFP (485 nm) for identification of N-glycanase 1 deficient fibroblasts | A congenital disorder of deglycosylation: Biochemical characterization of N-glycanase 1 deficiency in patient fibroblasts. Glycobiology (2015) 25(8):836–844 | P He, JE Grotzke, HH Freeze et al. | 25900930 | 2015 |
| High-Content Screening | SPECTRA X | Excitation of Alexa Fluor 488 and Alexa Fluor 568 transferrin conjugates for quantitative imaging of cellular transferrin content | Quantitative analysis of transferrin cycling by automated fluorescence microscopy. Methods Mol Biol (2015) 1270:365–378 | DT Hirschmann, CA Kasper, M Spiess | 25702129 | 2015 |
| Immunofluorescence Microscopy | SPECTRA | High-resolution imaging of chromatin organization combining multiplexed immunostaining, fluorescence in situ hybridization (FISH) and array tomography (AT) | A high-resolution imaging approach to investigate chromatin architecture in complex tissues. Cell (2015) 163(1):246–255 | MW Linhoff, SK Garg, G Mandel | 26406379 | 2015 |
| Immunofluorescence Microscopy | SOLA SE | (1) Excitation of Alexa Fluor 488, Alexa Fluor 568 and Alexa Fluor 647 labels. (2) Photoconversion of mEos2 (395 nm) | Talin determines the nanoscale architecture of focal adhesions. Proc Natl Acad Sci U S A (2015) 112(35):E4864–E4873 | J Liu, Y Wang, P Kanchanawong et al. | 26283369 | 2015 |
| Immunofluorescence Microscopy | SOLA SE | Fluorescence immunohistochemical visualization of biomarkers in stem cell-derived intestinal epithelia | Cloning and variation of ground state intestinal stem cells. Nature (2015) 522:173–178 | X Wang, Y Yamamoto, W Xian et al. | 26040716 | 2015 |
| Intravital Imaging | SPECTRA X | Intraoperative indocyanine green (ICG) fluorescence imaging of tumors | Quantification of tumor fluorescence during intraoperative optical cancer imaging. Sci Rep (2015) 5:16208 | RP Judy, JJ Keating, S Singhal et al. | 26563091 | 2015 |
| Intravital Imaging | SPECTRA X | Excitation of Alexa Fluor 488– and Alexa Fluor 647–labeled antibodies for imaging fibrin generation and platelet accumulation in mice | Novel mouse hemostasis model for real-time determination of bleeding time and hemostatic plug composition. J Thromb Haemost (2015) 13(3):417–425 | TM Getz, R Piatt, W Bergmeier et al. | 25442192 | 2015 |
| Live Cell Microscopy | SOLA SE | Excitation of GFP-ALADIN and mCherry–α-tubulin expressed in Drosophila S2 cells | The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation. Mol Biol Cell (2015) 26(19):3424–3438 | S Carvalhal, SA Ribeiro, ER Griffis et al. | 26246606 | 2015 |
| Live Cell Microscopy | SPECTRA X | Excitation of GFP and RFP for imaging of mitochondrial fusion | Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nat Commun (2015) 6:8472 | DG Phinney, M Di Giuseppe, LA Ortiz et al. | 26442449 | 2015 |
| Live Cell Microscopy | SPECTRA | Excitation of luminescent temperature sensor europium (III) thenoyltrifluoroacetonate (Eu-TTA) at 377 nm | Triggering of high-speed neurite outgrowth using an optical microheater. Sci Rep (2015) 5:16611 | K Oyama, V Zeeb, S Ishiwata et al. | 26568288 | 2015 |
| Live Cell Microscopy | SPECTRA X | Imaging of photoinduced lysosome damage in C. neoformans–infected bone marrow–derived macrophages | Cryptococcus neoformans-induced macrophage lysosome damage crucially contributes to fungal virulence. J Immunol (2015) 194(5):2219–2231 | MJ Davis, AJ Eastman, MA Olszewski et al. | 25637026 | 2015 |
| Materials Science | SOLA SE | Imaging of single quantum dots (QDs) on silver plasmonic resonators | Wedge waveguides and resonators for quantum plasmonics. Nano Lett (2015) 15(9):6267–6275 | SJ Kress, FV Antolinez, DJ Norris et al. | 26284499 | 2015 |
| Microfluidics | SPECTRA X | Evaluating the color of thermochromic liquid crystal (TLC) fluid flow tracers | Simultaneous three-dimensional temperature and velocity field measurements using astigmatic imaging of non-encapsulated thermo-liquid crystal (TLC) particles. Lab Chip (2015) 15:660–663 | R Segura, M Rossi, CJ Kähler et al. | 25431101 | 2015 |
| Microscopy Technique Development | SPECTRA X | Validation of a computational method for illumination uniformity correction | CIDRE: an illumination-correction method for optical microscopy. Nat Methods (2015) 12(5):404–406 | K Smith, Y Li, P Horvath et al. | 25775044 | 2015 |
| Optogenetics | SOLA SE | (1) Stimulation of ChR2 (470 nm) and Chrimson (615 nm) photoproteins. (2) Excitation of GCaMP3 Ca2+ sensor. | Feedback from network states generates variability in a probabilistic olfactory circuit. Cell (2015) 161(2):215–227. | A Gordus, N Pokala, CI Bargmann et al. | 25772698 | 2015 |
| Optogenetics | SPECTRA X | Optical activation of ChR2-expressing neurons (475/28 nm; 10 ms light pulses at 19 mW/mm2) | A sexually dimorphic hypothalamic circuit controls maternal care and oxytocin secretion. Nature (2015) 525(7570):519–522 | N Scott, M Prigge, T Kimchi et al. | 26375004 | 2015 |
| Optogenetics | SPECTRA X | Identification of melanopsin-based responses in photosensitive retinal ganglion cells using alternating blue (438 nm) and yellow (575 nm) stimuli | Melanopsin-derived visual responses under light adapted conditions in the mouse dLGN. PLoS ONE (2015) 10(3):e0123424 | KE Davis, CG Eleftheriou, RJ Lucas et al. | 25822371 | 2015 |
| Optogenetics | SOLA SE | Photoactivation of CRY2-CIB1 heterodimerization | Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol (2015) 22(5):671–682 | L Duan, D Che, B Cui et al. | 25963241 | 2015 |
| Photochemistry | SPECTRA | Irradiation of ruthenium polypyridyl sensitizer at 445 nm for photoelectrochemical water splitting | Visible photoelectrochemical water splitting into H2 and O2 in a dye-sensitized photoelectrosynthesis cell. Proc Natl Acad Sci U S A (2015) 112(19):5899–5902 | L Alibabaei, BD Sherman, TJ Meyer et al. | 25918426 | 2015 |
| Spectral Imaging | SPECTRA X | Integrated quantitative diffuse reflectance imaging and hyperspectral fluorescence imaging of protoporphyrin IX (PpIX) | Quantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection. Biomed Opt Express (2015) 6(12):4923–4933 | M Sibai, I Veilleux, BC Wilson et al. | 26713206 | 2015 |
| Confocal Microscopy | SPECTRA X | Excitation of mCherry for confocal imaging of telomere clustering using X-Light spinning disk system | Interchromosomal homology searches drive directional ALT telomere movement and synapsis. Cell (2014) 159(1):108–121 | NW Cho, RL Dilley, RA Greenberg et al. | 25259924 | 2014 |
| High-Content Screening | SPECTRA X | Excitation of Hoechst 33342 (377 nm), YOYO-1 (482 nm), MitoTracker Orange (543 nm) and DyLight 649 (650 nm) in 4-color multiplex hepatotoxicity assay | A micropatterned hepatocyte coculture model for assessment of liver toxicity using high-content imaging analysis. Assay Drug Dev Technol (2014) 12(1):16–27 | OJ Trask, A Moore, EL LeCluyse | 24444127 | 2014 |
| Immunofluorescence Microscopy | SOLA SE | Visualization of dual immunofluorescence labeling in mouse pancreatic islets | Adipsin Is an adipokine that Improves beta cell function in diabetes. Cell (2014) 158(1):41–53 | JC Lo, S Ljubicic, BM Spiegelman et al. | 24995977 | 2014 |
| Live Cell Microscopy | SPECTRA X | Excitation of PercevalHR ATP/ADP sensor at 438 nm and 482 nm | Imaging changes in the cytosolic ATP-to-ADP ratio. Methods Enzymol (2014) 547:355–371 | M Tantama, G Yellen | 25416365 | 2014 |
| Live Cell Microscopy | SOLA SE | 24-hour time-lapse imaging of promoter-dependent GFP expression in E. coli | Quorum-sensing crosstalk-driven synthetic circuits: from unimodality to trimodality. Chem Biol. (2014) 21(12):1629–1638 | F Wu, DJ Menn, X Wang | 25455858 | 2014 |
| Live Cell Microscopy | SPECTRA | High-speed (50 fps) imaging of GFP (ex = 470 nm) and mKate2 (ex = 555 nm) transgenic C. elegans | Non-microfluidic methods for imaging live C. elegans. Methods (2014) 68(3):542–547 | CJ Luke, JZ Niehaus, SC Watkins et al. | 24836996 | 2014 |
| Live Cell Microscopy | SPECTRA X | Calcium imaging in cardiac myocytes using fluo-4 (ex = 470 nm) | Cardiac CD47 Drives Left Ventricular Heart Failure Through Ca2+‐CaMKII‐Regulated Induction of HDAC3. J Am Heart Assoc. (2014) 3(3): e000670 | M Sharifi-Sanjani, AH Shoushtari, JS Isenberg et al. | 24922625 | 2014 |
| Microfluidics | SPECTRA X | 470 nm excitation of GFP for time-lapse imaging of cells in microfluidic networks | Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis. Nat Commun (2014) 5:4250 | O Frey, PM Misun, A Hierlemann et al. | 24977495 | 2014 |
| Microfluidics | SOLA SM | Imaging of binary micropatterns of Alexa Fluor 488– and Alexa Fluor 555–labeled antibodies deposited on glass surfaces | Hierarchical hydrodynamic flow confinement: efficient use and retrieval of chemicals for microscale chemistry on surfaces. Langmuir (2014) 30(12):3640–3645 | J Autebert, A Kashyap, GV Kaigala et al. | 24625080 | 2014 |
| Multimode Imaging | SPECTRA | Optimization of GFP fluorescence in dehydrated resin sections for correlative light and electron microscopy | Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells. Ultramicroscopy (2014) 143:3–14 | CJ Peddie, K Blight, LM Collinson et al. | 24637200 | 2014 |
| Optogenetics | SOLA SE | Transient (~100 ms) stimulation of ChR2 (460 –490 nm) in zebrafish hair cells | Optical stimulation of zebrafish hair cells expressing channelrhodopsin-2. PLoS ONE (2014) 9(5): e96641 | BD Monesson-Olson, J Browning-Kamins, JG Trapani et al. | 24791934 | 2014 |
| Optogenetics | SPECTRA | Stimulation (ChR2, 475 nm) and inhibition (Arch-T, 549 nm) of melanin-concentrating hormone (MCH) producing neurons | Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation. J Neurosci (2014) 34(20):6896–6909 | T Tsunematsu, T Ueno, A Yamanaka et al. | 24828644 | 2014 |
| Optogenetics | SPECTRA X | Activation (475 nm) and deactivation (632 nm) of bi-stable light-activated chloride channel SwiChR | Structure-guided transformation of channelrhodopsin into a light-activated chloride channel. Science (2014) 344(6182):420–424 | A Berndt, SY Lee, K Deisseroth et al. | 24763591 | 2014 |
| Optogenetics | SPECTRA X | Stimulation of light-activated chloride channel ChloC (476 nm) | Conversion of channelrhodopsin into a light-gated chloride channel. Science (2014) 344(6182):409–412 | J Wietek, JS Wiegert, P Hegemann et al. | 24674867 | 2014 |
| Optogenetics | SPECTRA X | Activation of ChR2 and NpHR with cyan (475 nm) and yellow (586 nm) light respectively in mouse dorsal root ganglion neurons | Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice. Nature Biotech (2014) 32(3):274–278 | SM Iyer, KL Montgomery, SL Delp et al. | 24531797 | 2014 |
| Confocal Microscopy | SPECTRA X | Excitation of Rhodamine PE (560 nm) and Alexa Fluor 647 (628 nm) for confocal imaging using X-Light spinning disk system | Phosphoinositides and membrane curvature switch the mode of actin polymerization via selective recruitment of toca-1 and Snx9. Proc Natl Acad Sci U S A (2013) 110(18):7193–7198 | JL Gallop, A Walrant, MW Kirschner et al. | 23589871 | 2013 |
| FRET | SPECTRA | Excitation of GFP (donor) at 472 nm and mCherry (acceptor) at 543 nm | A sensitized emission based calibration of FRET efficiency for probing the architecture of macromolecular machines. Cell Mol Bioeng (2013) 6(4):369–382 | A Joglekar, R Chen, J Lawrimore | 24319499 | 2013 |
| Live Cell Microscopy | SOLA SE | Pulsed excitation (450-490 nm) of GCaMP calcium sensor to limit phototoxic damage in live C. elegans nematodes | High-throughput imaging of neuronal activity in Caenorhabditis elegans. Proc Natl Acad Sci U S A (2013) 110(45):E4266-4273 | J Larsch, D Ventimiglia, DR Albrecht et al. | 24145415 | 2013 |
| Live Cell Microscopy | SOLA SE | Imaging of time-dependent GFP (ex = 472 nm) and mCherry (ex = 562 nm) expression in yeast | Engineering of regulated stochastic cell fate determination. Proc Natl Acad Sci U S A (2013) 110(26):10610–10615 | M Wu, RQ Su, X Wang et al. | 23754391 | 2013 |
| Optogenetics | SPECTRA | Transient (1–200 ms) stimulation of ChR2 (475 nm) in rat cortical neurons | Role of electrical activity in horizontal axon growth in the developing cortex: a time-lapse study using optogenetic stimulation. PLoS ONE (2013) 8(12):e82954 | O Malyshevskaya, Y Shiraishi, N Yamamoto et al. | 24376616 | 2013 |
| Optogenetics | SPECTRA | Optogenetic inhibition of Arch-T expressing neurons (542 nm) | Long-lasting silencing of orexin/hypocretin neurons using archaerhodopsin induces slow-wave sleep in mice. Behav Brain Res (2013) 255:64–74 | T Tsunematsu, S Tabuchi, A Yamanaka et al. | 23707248 | 2013 |
| FRET | SPECTRA | Excitation of cpCerulean (donor) at 433 nm and cpVenus (acceptor) at 515 nm | Orientation-based FRET sensor for real-time imaging of cellular forces. J Cell Sci (2012) 125(3):743–750 | F Meng, F Sachs | 22389408 | 2012 |
