Light BYTES – November 2020: Intensity Control Linearity in Lumencor’s New Generation of Light Engines

Lumencor’s new Generation of Light Engines: Intensity Control linearity

AURA, RETRA, SPECTRA, CELESTA and ZIVA light engines, as well as the newly refreshed SOLA light engine for 2021, incorporate on-board microprocessors, providing impressive advances in control and monitoring capabilities. One such advancement is linear intensity control. Each of these illuminators generate optical power that has a precisely linear relationship to intensity settings across all colors, providing more quantitive and predictable responses for users. In the case of white light, constant color temperature is achievable regardless of output power. Just another reason customers who care about performance come to Lumencor for solid state illumination.


Download the PDF of Lumencor lightBYTES: November 2020

Parallel Light Engine Performance monitoring Using the Onboard Control GUI

Parallel Light Engine Performance monitoring Using the Onboard Control GUI

AURA, CELESTA, RETRA, SPECTRA, and ZIVA Light Engines incorporate a control GUI accessed through a web browser via an ethernet connection.  Image acquisition applications used to control the light engine though connection of either the USB or RS232 serial ports can be run in parallel with ethernet-connected control GUI to aid in trouble-shooting.  As shown in Figure 1, this allows the light engine to be controlled by the image acquisition software, while the GUI serves as a passive monitor of the light engine status.

Figure 1.  Screenshots of parallel operation of image acquisition software (NIS Elements, left) and CELESTA Light Engine Control GUI (right).  A. NIS Elements command to turn on red light output at 21.7% is inoperative.  Examination of the GUI display reveals that this is due to an open interlock condition (e.g. no optical fiber connected to the light engine output port).  B. After closing the interlock, the same NIS Elements command results in light output, indicated by the filled red channel radio button and non-zero output power reading in the GUI.

The control GUI displays many types of information pertinent to the performance of the light engine that are not accessible in current releases of most image acquisition software packages.   These include:

  • Real-time light output power readouts
  • Standby mode status
  • Light engine operating software error messages
  • Humidity/dew point data
  • Serial port configuration
  • TTL port configuration
  • Cumulative run time data

In cases where the PC being used for image acquisition control has a single ethernet port that is dedicated to internet access, a USB-to-ethernet adapter (Figure 2) can be used for connection to the light engine control GUI.  USB-to-ethernet adapters are readily available from online vendors for less than $20.

Figure 2. USB-to-ethernet adapter

 

 

 

 

 

Optogenetics

Optogenetics

Optogenetics

Neuroscience is the multidisciplinary branch of biology in which the fundamental properties of neurons, the organization of neural networks, and their behavioral and sensory functional products are studied. Because the functions of neural networks are inextricably linked to their spatial organization, neuroscience research is heavily dependent on a variety of imaging techniques. For cellular and tissue level investigations, fluorescence microscopy is the most instructive and widely applicable of these techniques. Optogenetic techniques provides researchers with ways of externally modulating neuronal network function using light as an intermediary.

To find out more about Lumencor light engines that are suitable for Neuroscience studies, please contact us.

Find out more about Lumencor light engines that are suitable for Optogenetics studies.

Contact Us

 

Optogenetics

Neuroscience

Neuroscience

Neuroscience is the multidisciplinary branch of biology in which the fundamental properties of neurons, the organization of neural networks, and their behavioral and sensory functional products are studied. Because the functions of neural networks are inextricably linked to their spatial organization, neuroscience research is heavily dependent on a variety of imaging techniques. For cellular and tissue level investigations, fluorescence microscopy is the most instructive and widely applicable of these techniques. Optogenetic techniques provides researchers with ways of externally modulating neuronal network function using light as an intermediary.

Find out more about Lumencor light engines that are suitable for Neuroscience studies.

Contact Us

 

Light BYTES: December 2016

AURA Light Engine® for Ca2+ Ratio Imaging

 

Fluorescence excitation spectra of fura-8 Ca2+ indicator showing 360/28 nm and 395/25 nm excitation bandpasses supplied by Lumencor’s AURA light engine

Fluorescence excitation spectra of fura-8 Ca2+ indicator showing 360/28 nm and 395/25 nm excitation bandpasses supplied
by Lumencor’s AURA light engine


 
Ratio imaging of intracellular calcium has long been an important technique in cell biology, neuroscience and related fields. Excitation ratio imaging compensates for variations of indicator dye concentration within cells and between cells that might otherwise be interpreted as calcium level changes.

The preferred indicator dyes for Ca2+ ratio imaging are typically fura-2 and the recently developed red-shifted analog fura-8. Excitation ratio imaging is conventionally implemented using a white light source in combination with mechanically alternated filters to select the desired excitation wavelengths (340 and 380 nm for fura-2, 360 and 400 nm for fura-8).

Lumencor’s AURA light engine generates these excitation outputs from two discrete electronically controlled solid-state light sources. The AURA light engine can accommodate up to five sources, providing additional outputs for optogenetics or GFP/mCherry excitation.

Our RETRA light engine can be configured with 360/28 nm and 395/25 nm outputs for customers requiring a dedicated two-channel excitation source. Electronic alternation of excitation wavelengths provided by AURA or RETRA light engines is faster and more reproducible than mechanical methods. In turn, this allows higher-speed data acquisition, providing increased temporal resolution for recording elementary processes in cell physiology.

For further information, download our calcium ratio imaging application note.

Download the PDF of Light BYTES: December 2016.

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14940 NW Greenbrier Parkway | Beaverton, OR 97006 USA | 503.213.4269 | info@lumencor.com