Light BYTES: March 2020

Lumencor’s Best Practices
During COVID-19

Lumencor’s manufacturing operations remain healthy and active. While daily operations are inevitably constrained by measures taken to foster good health and minimize the transmission of COVID-19, productivity remains high. Minimal component shortages have developed as shelter-in-place practices have dampened our suppliers’ activities. However, we expect our supply chain will likely suffer additional shortages. Lumencor’s practices now include the quarantine of all receivables as our first commitment is to ensure the safety of our employees while safeguarding their work. In some cases, that may mean that our deliveries to customers will be delayed. We will do our best to inform all customers with pending orders of changes to anticipated shipment dates. Please notify us of any urgency around your order and we will do our very best to assist.

Please be assured that we are watching and managing your business with great care during the current emergency. Please contact to our sales, customer service or technical support representatives if you have any questions or wish to discuss deliverables for your organization’s needs.


A Spectacular Triple Play: LIDA Light Engine®, NIS Elements and sCMOS Cameras for Color Light Microscopy

For histologists, clinical pathologists and anyone seeking improvements in the speed, sensitivity and precision of transmitted light microscopy, Lumencor’s LIDA light engine in combination with Nikon NIS Elements software enable high-speed color imaging data without the need for a dedicated color camera. Instead, monochrome images generated by sequential triggering of the LIDA’s red, green and blue light sources by a sCMOS camera are processed by NIS Elements, delivering video-rate color output. These capabilities allow rapid and fully automated imaging of large tissue sections, as illustrated below. The software also enables convenient switching between camera-synchronized RGB illumination and white-light illumination for ocular viewing. Our application note RGB Color Imaging using the LIDA Light Engine and NIS Elements outlines hardware set-up for Hamamatsu ORCA-Flash4.0 and Andor Zyla sCMOS cameras and provides instructions for image acquisition control using NIS Elements software.

Color image of a 1.5 cm x 1 cm section of adenocarcinoma from human breast acquired using Lumencor’s LIDA light engine and NIS Elements software. Image courtesy of Dr. Michael Weber (Harvard Medical School).

 

Lumencor’s LIDA light engine mounted on the transillumination port of a Nikon Ti2 microscope with Andor Zyla 5.5 megapixel sCMOS camera


Submit your best microscopy images in Lumencor’s 2020 Earth Day Light Microscopy Competition

Winning submissions have the potential to earn you up to $10,000 worth of high tech, solid state lighting.

 


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Light BYTES: February 2020

Precise power regulation on each of
Lumencor’s newest and brightest light engines:
AURA®, SPECTRA®, CELESTA®, and ZIVA light engines®

In addition to high power and intense brightness, output power regulation is one of the many advanced control features incorporated in Lumencor’s next generation products: SPECTRA, AURA, CELESTA, and ZIVA light engines. To use power regulation, a desired power reference value in milliwatts is entered in the onboard control GUI, as shown in the attached link. To activate power regulation, click the padlock icon next to the reference power value. Gray shading of the padlock icon and the reference power value shows that power regulation is active for the selected output channel. When power regulation is active, the intensity setting for the channel is controlled by the onboard microprocessor, based on feedback from the light engine’s reference photodiode array. The microprocessor continuously adjusts the intensity setting so that the output power matches the power reference value set by the user.

Output power regulation settings in the CELESTA light engine control GUI

Performance of the output power regulation feedback system of a SPECTRA light engine is illustrated below. The response time of the feedback system is approximately one second. Output power regulation allows users to eliminate variations in light output due to temperature fluctuations and other environmental factors in photometric and quantitative imaging applications where reproducibility and accuracy are essential.

Teal (510/25 nm) channel output from a SPECTRA light engine with and without power regulation. Power output from the light guide was monitored with a Coherent PowerMax II-TO power meter with model PM3 thermopile detector.


Download the PDF of Light BYTES: February 2020

CELESTA and SPECTRA III Standby Mode

CELESTA® and SPECTRA III light engines® with operating software version 2.1.19 and above have a standby mode to conserve power during periods when no active light output generation is required. The light engine automatically switches into standby mode after a set latency period (i.e. after the last light output = OFF command was issued). The default latency period is 300 seconds (5 minutes), however this can be temporarily reset by the user. The characteristics of standby mode registered by the onboard control GUI are shown in the adjacent screenshot. Standby mode is also marked by an automatic shut-off of the main cooling fan. Standby mode automatically terminates when the next light output = ON command is issued.

CELESTA light engine® Control GUI in standby mode:

  1. Light output = OFF in all channels (all radio buttons unfilled).
  2. Status indicator (STS) = red.
  3. Standby mode indicator. In the latency period after the last light output = OFF command, this display reads “Standby in # seconds” where # is between zero and 300 (default) seconds. When light output is active, this display reads “Active”.
  4. Operating software version number.
  5. Portal to command line interface (for resetting the latency period from its default value of 300 seconds).

Light BYTES: November 2019

ZIVA® versus CELESTA®: It’s all about the fiber

In 2018, Lumencor introduced the CELESTA light engine incorporating 7 individually addressable laser light sources. The ZIVA light engine, introduced at the 2019 Society for Neuroscience meeting in Chicago, while similar in some respects, is distinctive in being designed to couple into smaller bore optical fibers.

The output of the ZIVA light engine is suitable for structured illumination microscopy (SIM) and other super-resolution microscopy techniques. In these applications, it provides an alternative to more costly and hard-to-align single mode laser sources. The larger illumination field of the CELESTA light engine is preferred for applications such as spinning disk confocal microscopy, MERFISH or smFISH.


Common Features of ZIVA and CELESTA Light Engines

  • Same 7 laser lines (405 nm, 446 nm, 477 nm, 520 nm, 546 nm, 638 nm, 749 nm)
  • Same compact 15 cm x 35 cm footprint
  • Same onboard microprocessor-based control and feedback interface
  • Same laser safety interlock configuration

100X widefield (a) and structured illumination microscopy (b) images of actin (green) and mitochondria (orange) in fixed bovine pulmonary endothelial cells. From Pospíšil et al, GigaScience (2018) 8:1–12


Download the PDF of Light BYTES: November 2019

Light BYTES: September 2019

SOLA® for the win!

This year Lumencor launched the Light Microscopy Imaging Competition in celebration of Earth Day to highlight Lumencor’s commitment to manufacturing bright, clean, and mercury-free light engines. We were impressed with the quality and breadth of applications in the submissions we received. Without further ado here are the winners for Lumencor’s 2019 Earth Day Light Microscopy Imaging Competition:

 

1st Place – Robert Zucker
EPA, Research Triangle Park, NC
PRIZE: NEW SOLA SE U-nIR Light engine

ARPE 19 cell treated with 75nm silver nanoparticles that are coated with PVP Combination of darkfield image (white nanoparticles) and fluorescence image of nuclei (blue), Golgi (green), and microtubules (red). Observation with a using a 60X plan Fluor lenses and a SOLA light engine®. The image was processed using Nikon deconvolution algorithms.


2nd Place – Glyn Nelson
University of Newcastle, UK
PRIZE: $1000 off voucher

Image of a mosquito larva’s ‘mustache’, the fine brush hairs on the side of their mouth. Z stack deconvolved and rendered in Huygens. Larva fixed in PFA and mounted in glycerol. Imaged with a Leica DMi8 equipped with a 40X oil 1.3 NA objective, GFP filter cube, Hamamatsu Flash4 v2 camera and a SOLA SE light engine.


3rd Place – Abdul Mohammed
Ultivue, Cambridge, MA
PRIZE: $500 off Voucher

16-plex biomarker profiling of Non-Small Cell Lung Cancer tissue (CD3, CD4, CD45RO, CD68, CD163, CD8, CD11c, CD20, PD1, PDL1, Cytokeratin, LAG3, FoxP3, Ki67, GranzymeB and MHCII). Nuclear counterstain shown in blue. The slide was processed using Ultivue’s InSituPlex technology and imaged on a fluorescence microscope using a 20X objective and SPECTRA X light engine®.



These images clearly demonstrate the quality of data attainable by using Lumencor’s solid state light engines. For more information on how Lumencor light engine can help you in you illumination needs please contact us.


Download the PDF of Light BYTES: September 2019

Packaging Guidance for Shipping Light Engines

Occasionally it will be necessary to ship a light engine back to our factory in Beaverton for service. On these occasions, you will receive an e-mail containing a return material authorization (RMA) and shipping instructions from our technical support department. Among those instructions are “Please ensure that the light engine is securely packaged for shipment, if possible in the original transit box in which it was delivered”. Secure and effective packaging is important in order to avoid additional costs in time and money for repairing damage incurred during transit. Here we offer some more detailed guidance on packaging light engines for shipment.

It is recommended to follow Lumencor’s packaging for new light engine shipments as closely as possible. The performance of this packaging has been proven in the course of thousands of shipments all over the world. An example is shown in the adjacent photo. Particularly important are the rigid expanded foam inserts. These prevent movement of the light engine within the box during transit. Bubble wrap, air pillows or loose packing material such as styrofoam “peanuts” are NOT effective substitutes and should NOT be used. Other important packaging tips include:

  • Detach the liquid light guide from the light engine and from its collimating adapter. Coil it loosely in the mylar pouch that it came in, or in a 2-gallon (33 x 38 cm) “ziploc” bag. Place the bag containing the light guide above the light engine in the shipping box, separated by a layer of packing material.
  • Do NOT use fibrous packing material that is liable to disintegrate during transit. Small pieces of packing material debris may end up deposited on the internal electronics and optics of the light engine.
  • Disconnect the DC power supply from the light engine and pack it separately inside the shipping box.

For additional guidance in preparing light engine shipments, please e-mail your questions to techsupport@lumencor.com.

lumencor
14940 NW Greenbrier Parkway | Beaverton, OR 97006 USA | 503.213.4269 | info@lumencor.com