Recommended Operating Conditions for CELESTA, SPECTRA and ZIVA Light Engines®

Recommended Operating Conditions for CELESTA, SPECTRA and ZIVA Light Engines®

 

To support the long-term stability of the laser light sources in CELESTA, SPECTRA and ZIVA light engines it is recommended that they should be operated only in environments where the dew point is below 15ºC. For reference, at a typical room temperature of 24ºC, a dew point of 15ºC corresponds to 57% humidity. The current dew point inside the light engine, calculated from onboard temperature and humidity sensors, is displayed on the settings page of the onboard control GUI.

Standby Mode, previously described in the October 2019 issue of Light Reading, is another control system designed to support the long-term stability of the laser light sources. Consequently, users writing their own light engine control software are strongly advised to NOT programmatically disable standby mode.

 

 

If operational situations arise where it is necessary to avoid the onset of standby mode during a data acquisition process, please contact techsupport@lumencor.com. We will be happy to work with you and our software engineering team to devise appropriate solutions.

 

Download the PDF of Lumencor Light Reading: July 2020

CELESTA and SPECTRA Standby Mode

CELESTA® and SPECTRA light engines® with operating software version 2.1.19 and above have a standby mode to conserve power during periods when no active light output generation is required. The light engine automatically switches into standby mode after a set latency period (i.e. after the last light output = OFF command was issued). The default latency period is 300 seconds (5 minutes), however this can be temporarily reset by the user. The characteristics of standby mode registered by the onboard control GUI are shown in the adjacent screenshot. Standby mode is also marked by an automatic shut-off of the main cooling fan. Standby mode automatically terminates when the next light output = ON command is issued.

CELESTA light engine® Control GUI in standby mode:

  1. Light output = OFF in all channels (all radio buttons unfilled).
  2. Status indicator (STS) = red.
  3. Standby mode indicator. In the latency period after the last light output = OFF command, this display reads “Standby in # seconds” where # is between zero and 300 (default) seconds. When light output is active, this display reads “Active”.
  4. Operating software version number.
  5. Portal to command line interface (for resetting the latency period from its default value of 300 seconds).

Packaging Guidance for Shipping Light Engines

Occasionally it will be necessary to ship a light engine back to our factory in Beaverton for service. On these occasions, you will receive an e-mail containing a return material authorization (RMA) and shipping instructions from our technical support department. Among those instructions are “Please ensure that the light engine is securely packaged for shipment, if possible in the original transit box in which it was delivered”. Secure and effective packaging is important in order to avoid additional costs in time and money for repairing damage incurred during transit. Here we offer some more detailed guidance on packaging light engines for shipment.

It is recommended to follow Lumencor’s packaging for new light engine shipments as closely as possible. The performance of this packaging has been proven in the course of thousands of shipments all over the world. An example is shown in the adjacent photo. Particularly important are the rigid expanded foam inserts. These prevent movement of the light engine within the box during transit. Bubble wrap, air pillows or loose packing material such as styrofoam “peanuts” are NOT effective substitutes and should NOT be used. Other important packaging tips include:

  • Detach the liquid light guide from the light engine and from its collimating adapter. Coil it loosely in the mylar pouch that it came in, or in a 2-gallon (33 x 38 cm) “ziploc” bag. Place the bag containing the light guide above the light engine in the shipping box, separated by a layer of packing material.
  • Do NOT use fibrous packing material that is liable to disintegrate during transit. Small pieces of packing material debris may end up deposited on the internal electronics and optics of the light engine.
  • Disconnect the DC power supply from the light engine and pack it separately inside the shipping box.

For additional guidance in preparing light engine shipments, please e-mail your questions to techsupport@lumencor.com.

Device Drivers for SPECTRA III, CELESTA, & AURA III light engines

The latest nightly builds of MicroManager 1.4.23 now include device drivers for SPECTRA III®, CELESTA® and AURA III® light engines. The basic set-up in the MicroManager Hardware Configuration Wizard is shown in the screenshot below.

Note that the GEN3 driver uses STANDARD mode communications. The light engine serial port addressed by MicroManager must therefore be set to STANDARD mode on the settings page of the Web GUI.

Other image acquisition software packages that now support SPECTRA III, CELESTA, and AURA III light engines are:

  • NIS Elements (Version 5.11 and above)
  • MetaMorph

For updates on controlling SPECTRA III, CELESTA, and AURA III light engines using other software packages, please email our Technical Support group at techsupport@lumencor.com.

New LIDA Light Engine

LIDA 4-PANEL: Red, green and blue grayscale and composite RGB color images of mammalian pyloric stomach (LIDA light engine, Nikon Ti microscope, 10X objective)

LIDA 4-PANEL: Red, green and blue grayscale and composite RGB color images of mammalian pyloric stomach (LIDA light engine, Nikon Ti microscope, 10X objective)

Our new LIDA light engine® is designed from the ground up to work hand-in-hand with the latest monochrome cameras to generate RGB color transmitted light images with unprecedented sensitivity, spatial resolution, speed and color fidelity. Many capabilities and features are integrated into a small package mounted directly to the microscope’s transmitted light illumination port. TTL gating of LIDA’s three solid state light sources synchronized with camera exposure generates color transmitted light images in one electronically controlled, high-speed sequence. For more information on the LIDA light engine, please speak to your Lumencor sales representative or contact us at info@lumencor.com.

New Improved Liquid Light Guides

2248-LUM.Web_0316.Blog_LiquidLightGuides

SPECTRA X: light engine delivered output delivered through original (10-10084A) and improved (10-10084B) 3mm diameter liquid light guides (LLG).

Liquid light guides (LLG) provide flexible and convenient conduits for light delivery to fluorescence microscopes and other bioanalytical instruments. In addition to isolating the microscope from any heat and vibration associated with the light source, passage through a light guide acts to randomize spatial inhomogeneity in the light source output.

One disadvantage, however is that up to 30% of the original source output can be lost in the process of coupling into, and transmission through, the light guide. We have recently modified the design of our liquid light guides to minimize these losses. The result is liquid light guides that deliver about 10% more output to the microscope than their predecessors.

Click here, for more technical information on Lumencor’s liquid light guides, optical fibers and microscope input collimators.

lumencor
14940 NW Greenbrier Parkway | Beaverton, OR 97006 USA | 503.213.4269 | info@lumencor.com