Light BYTES – May 2021: Epi-illuminators for CELESTA Light Engines® in Super-Resolution Imaging

Epi-illuminators for CELESTA Light Engines® in Super-Resolution Imaging

Super-resolution imaging of RNA transcripts using multiplex error robust fluorescence in situ hybridization (MERFISH) and related methods in combination with the CELESTA Light Engine® [1] requires higher levels of irradiance (mW/mm2) than can be obtained from Koehler illumination. To facilitate these applications, Lumencor has developed a series of epi-lluminators that provide uniform illumination over a smaller field of view but with much higher irradiance than typical Koehler illumination can provide. Epi-illuminators compatible with all major research microscope brands are available.

Reference
[1] JH Su, P Zheng, X Zhuang et al. Cell (2020) 182:1641–1659


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