Light BYTES – March 2020: LIDA Light Engine®, NIS Elements, and sCMOS Cameras for Color Light Microscopy

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A Spectacular Triple Play: LIDA Light Engine®, NIS Elements and sCMOS Cameras for Color Light Microscopy

For histologists, clinical pathologists and anyone seeking improvements in the speed, sensitivity and precision of transmitted light microscopy, Lumencor’s LIDA light engine in combination with Nikon NIS Elements software enable high-speed color imaging data without the need for a dedicated color camera. Instead, monochrome images generated by sequential triggering of the LIDA’s red, green and blue light sources by a sCMOS camera are processed by NIS Elements, delivering video-rate color output. These capabilities allow rapid and fully automated imaging of large tissue sections, as illustrated below. The software also enables convenient switching between camera-synchronized RGB illumination and white-light illumination for ocular viewing. Our application note RGB Color Imaging using the LIDA Light Engine and NIS Elements outlines hardware set-up for Hamamatsu ORCA-Flash4.0 and Andor Zyla sCMOS cameras and provides instructions for image acquisition control using NIS Elements software.

Color image of a 1.5 cm x 1 cm section of adenocarcinoma from human breast acquired using Lumencor’s LIDA light engine and NIS Elements software. Image courtesy of Dr. Michael Weber (Harvard Medical School).

Lumencor’s LIDA light engine mounted on the transillumination port of a Nikon Ti2 microscope with Andor Zyla 5.5 megapixel sCMOS camera


Submit your best microscopy images in Lumencor’s 2020 Earth Day Light Microscopy Competition

Winning submissions have the potential to earn you up to $10,000 worth of high tech, solid state lighting.



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