Light BYTES: May 2016

Better Red Than Dead…

In addition to savings of $500–$1000 per year in operating and maintenance costs, another major benefit of Lumencor’s SOLA light engines® over metal halide lamps is their superior output in the red region (>620 nm) of the visible spectrum. Images of selaginella autofluorescence, excited from 617–645 nm and detected under identical conditions, illustrate the improved image data quality delivered by the SOLA light engine.

Excitation at >620 nm provides advantages in terms of greater sample transparency, less generation of phototoxic reactive oxygen species and lower background signals compared to excitation at shorter wavelengths. Many red-excited fluorophores including Cy5, Alexa Fluor 647, ATTO 647N TO-PRO-3 and DRAQ5 have been developed to allow exploitation of these advantages.

Give your microscope a SOLA light engine upgrade today and look forward to brighter days ahead!

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Technical Details

  • Single band exciter 631/28 (CWL/FWHM). Semrock FF660-Di02 dichroic and FF01-692/40 emitter.
  • Nikon Ti microscope, 10X, 0.5 NA objective, Andor Zyla 5.5 camera, 50 ms exposures.
  • Power output at sample plane (10X, 0.5 NA objective) SOLA SE (100%) = 94 mW. 120 W metal halide (100 %) = 27 mW.
  • Sample: Autofluorescent longitudinal section of a strobilus from selaginella (heterosporous clubmoss).

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