Intravital imaging can be performed using a range of contrast mechanisms including fluorescence, magnetic resonance, ultrasound, and X-ray, sometimes in combination with one another. In all cases, the objective is to noninvasively characterize the morphology of a living organism at various levels of spatial resolution. Penetration of light into living tissues is limited to a few millimeters, maximally in the near-infrared wavelength range (650–900 nm). For regions of interest further from the surface, light must be delivered and retrieved through an endoscope. Lumencor’s solid-state illuminators are ideally suited with light sources to fulfill these and other technical specifications for intravital imaging applications.