Application Bibliography
| Application | Area Product | Reference | Date |
|---|---|---|---|
Validation of aiSEGcell protocol applied to time-lapse imaging of NF-kappa B signaling dynamics | High-Content Screening SPECTRA X Light Engine | aiSEGcell: User-friendly deep learning-based segmentation of nuclei in transmitted light images. PLoS Comput Biol (2024) 20:e1012361 D Schirmacher, U Armagan, T Schroeder et al. | 2024 |
Freestanding bilayer microscopy (FBM) of human mechanosensitive potassium channel (hTRAAK) in lipid bilayer membranes | Biophysics SPECTRA X Light Engine | Freestanding bilayer microscope for single-molecule imaging of membrane proteins. Sci Adv (2024) 10:eado4722 G Pérez-Mitta, Y Sezgin, R MacKinnon et al., | 2024 |
Iterative indirect immunofluorescence imaging (4i) of retinoblastoma (Rb) phosphorylation in MCF-10A human mammary epithelial cells | Immunofluorescence Microscopy SPECTRA X Light Engine | An intermediate Rb–E2F activity state safeguards proliferation commitment. Nature (2024) 631:424–431 Y Konagaya, D Rosenthal, T Meyer et al. | 2024 |
Optical control of protein dimerization via reversible photoisomerization of an azobenzene ligand | Optogenetics SPECTRA X Light Engine | 2024 | |
Imaging T-cell calcium signaling elicited by a biomimetic antigen-presenting system (bAPS) | Bioengineering SPECTRA X Light Engine | 2024 | |
In situ sequencing (ISS) for single-cell spatial expression profiling of the spatiotemporal cellular dynamics underlying autoimmune encephalomyelitis (EAE) evolution. | Transcriptomics SPECTRA X Light Engine | Cellular architecture of evolving neuroinflammatory lesions P Kukanja, CM Langseth, G Castelo-Branco et al. | 2024 |
Imaging intracellular neuropeptide concentrations using a genetically encoded sensor comprising vasoactive intestinal peptide receptor VPAC1 coupled to circularly permuted GFP | Pathology SPECTRA X Light Engine | Multisensory gamma stimulation promotes glymphatic clearance of amyloid. Nature (2024) 627:149–156 MH Murdock, CY Yang, LH Tsai et al. | 2024 |
Simultaneous 473 nm photstimulation of ChR2 and 585 nm excitation of genetically encoded voltage indicator Voltron fluorescence in murine acute brain slices. | Optogenetics SPECTRA X Light Engine | Voltage-Seq: all-optical postsynaptic connectome-guided single-cell transcriptomics. Nat Methods (2023) 10:1409-1416 V Csillag, MH Bizzozzero, J Fuzik et al. | 2023 |
1) Photoactivation of ChR2 (467–499 nm) and ACR1 (500–550 nm) in C. elegans. (2) Excitation of genetically encoded fluorescent calcium indicators and jRGECO1a and GCaMP. | Optogenetics SPECTRA X Light Engine | Neural engineering with photons as synaptic transmitters. Nat Methods M Porta-de-la-Riva, AC Gonzalez, M Krieg et al. | 2023 |
Detection of mechanosensory conformational rearrangements of PIEZO channels in HEK cells using cpGFP fluorescence | Microfluidics SPECTRA X Light Engine | 2023 | |
Temporally multiplexed imaging (TMI) using reversibly photoswitchable fluorescent proteins | Microscopy Technique Development SPECTRA X Light Engine | Temporally multiplexed imaging of dynamic signaling networks in living cells. Cell (2023) 186:1–17 Y Qian, OT Celiker, ES Boyden et al. | 2023 |
Imaging of genetically encoded voltage indicator (GEVI) JEDI-1P in dissociated rat cortical and hippocampal neurons | Live Cell Microscopy SPECTRA X Light Engine | 2023 | |
Maximum a posteriori probability structured illumination microscopy (MAP-SIM) of GFP–labeled coronal mouse brain slices | Superresolution Microscopy SPECTRA X Light Engine | 2023 | |
Imaging of GFP-tagged synaptic proteins in C. elegans | Intravital Imaging SPECTRA X Light Engine | 2023 | |
Photostimulation (470 nm, 30 mW/mm2) of Ziapin2 in human induced pluripotent stem cell-derived cardiomyocytes | Optogenetics SPECTRA X Light Engine | 2023 | |
Optogenetic inhibition using 513 nm light-gated potassium channels (kalium channelrhodopsins) in acute mouse brain slices | Optogenetics SPECTRA X Light Engine | All-optical physiology resolves a synaptic basis for behavioral timescale plasticity. Cell (2023) 186:543–559 LZ Fan, DK Kim, K Deisseroth et al. | 2023 |
Mapping of cellular heterogeneity in the developing human lung using hybridization-based in situ sequencing (HybISS) | Transcriptomics SPECTRA X Light Engine | A topographic atlas defines developmental origins of cell heterogeneity in the human embryonic lung. Nat Cell Biol (2023) 25:351–365 A Sountoulidis, SM Salas, C Samakovlis et al. | 2023 |
Photoelectrochemical (PEC) hydrogen generation in a particle suspension reactor (PSR). | Photochemistry SPECTRA X Light Engine | Water splitting with silicon p-i-n superlattices suspended in solution. Nature (2023) 614:270–274 TS Teitsworth, DJ Hill, JF Cahoon et al. | 2023 |
In situ fluorescence microscopy-guided focused ion beam (FIB) micromachining | Multimode Imaging SPECTRA X Light Engine | A cryogenic, coincident fluorescence, electron, and ion beam microscope. Elife (2022) 11:e82891 DB Boltje, JP Hoogenboom, S den Hoedt et al. | 2022 |
Cyclic immunofluorescence (CyCIF) detection of 40–60 protein targets in formalin-fixed paraffin-embedded (FFPE) human tissues | Immunofluorescence Microscopy SPECTRA X Light Engine | A framework for multiplex imaging optimization and reproducible analysis. Commun Biol (2022) 5:438 J Eng, E Bucher, JW Gray et al. | 2022 |
Imaging of GFP-cyclin-dependent kinase (CDK) and mitotic CDK activity biosensor, synCut3–mCherry in Schizosaccharomyces pombe fission yeast | Live Cell Microscopy SPECTRA X Light Engine | Core control principles of the eukaryotic cell cycle. Nature (2022) 607:381–386 S Basu, J Greenwood, P Nurse et al. | 2022 |
Identification of Parkinson’s disease-specific cellular phenotypes from high-content imaging datasets | High-Content Screening SPECTRA X Light Engine | 2022 | |
Hybridization-based in situ sequencing (HybISS) via direct RNA detection for identifying low abundance transcripts in mouse brain sections | Transcriptomics SPECTRA X Light Engine | Direct RNA targeted in situ sequencing for transcriptomic profiling in tissue. Sci Rep (2022) 12:7976 H Lee, SM Salas, M Nilsson et al. | 2022 |
Optogenetic induction of telomere coalescence | Optogenetics SPECTRA X Light Engine | Compartmentalization of telomeres through DNA- scaffolded phase separation. Dev Cell (2022) 57:277–290 A Jack, Y Kim, CP Brangwynne et al. | 2022 |
Imaging of labeled mRNA transcripts for validation of probe hybridization | Transcriptomics SPECTRA X Light Engine | 2022 |