Quantitative Fluorescence

Quantitative fluorescence microscopy techniques offer a researcher powerful tools to understanding physiological and pathological roles of molecular processes in living cells. For example, to understand regulation of protein expression, it is necessary to know the amounts of protein expressed as a function of time. However the fluorescence levels in a field of view depend not only on the amount of protein present, but also on external factors, including the excitation light intensity. Techniques that are based in fluorescence such as FLIM, FCS, FCCS, FRET, and FRAP aid in the measurement and quantification of data.

Find out more about Lumencor light engines that are suitable for Quantitative Fluorescence studies.

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