SEEING MORE WITH LESS
- Your job is to assist and advise researchers in the use of advanced microscopy techniques, correct?
- Yes, I am an imaging specialist at the Advanced Light Microscopy Core at The Jungers Center at Oregon Health Sciences University. It is a shared resource of high-end microscopy systems. I’m responsible for 10 microscope systems and support a user base of over 350 researchers and clinicians.
- How have Lumencor’s light engines impacted your work?
- We installed a Lumencor light engine as part of our wide-field deconvolution imaging system. It replaced a metal halide light source.There was so much more intensity with the light engine that the speed of analysis was dramatically improved. Our data acquisition rates (frames per second) increased by a factor of 2 for fixed samples and by a factor of 4 for live cell samples.Illumination necessary to achieve a good image was reduced from hundreds of milliseconds to tens of milliseconds. Anybody who has struggled with phototoxicity problems in live cell imaging experiments will readily appreciate the benefits of being able to limit light exposure.
- What are some other benefits that you and users of your facility have noticed?
- The illumination is even across the field of view through the eyepieces, which is larger than that of the camera detection area. An additional attraction is the rapid electronic switching between excitation channels, providing a further decrease in acquisition speed compared to microscopes equipped with mechanical filter wheels.
- Aurelie Snyder