Light Bytes: Application SpotLIGHT

Multiplexed imaging of gene expression using the CELESTA light engine

MERFISH (multiplex error robust fluorescence in situ hybridization) is an imaging technique that profiles cell populations based on the identification of thousands of RNA transcripts per cell.  The CELESTA light engine is an ideal and widely-adopted illumination source for this application.  In a recent paper published in Nature [1], Wheeler and co-workers used MERFISH imaging with a CELESTA light engine to quantify the expression of nine specific astrocyte and T-cell markers.  Five of the CELESTA light engine’s seven laser lines were used in the highly multiplexed MERFISH imaging protocol.  The overall objective of the research described in the paper was to characterize astrocyte populations that contribute to pathogenesis in a preclinical model of multiple sclerosis.

Reference
[1] MA Wheeler, IC Clark, FJ Quintana et al. Nature (2020) 578:593–5990

Download the PDF of Lumencor Light BYTES: Application SpotLIGHT

Light BYTES: May 2020

Independent Intensity and Pulse Width Control for Stroboscopic Illumination

Evaluation of photo-stimulation intensity dependence is often a necessary part of neuromodulation experimentation utilized in optogenetics studies [1]. The inherent stability and quantitative nature of Lumencor’s SPECTRA X light engine make it particularly well suited as the pulsed light source of choice for studies requiring pulse width and frequency of stroboscopic illumination analyses. Find more detail regarding this extremely stable, reproducible, and well-behaved data, as well as a specific reference in a recent Journal of Physiology publication by authors Kubota, Sidikejiang, and Seki, on Lumencor’s website.

Figure Description: Alternating cyan (485/25 nm, ~0.5 ms) and green (560/32 nm, ~3 ms) output pulses generated by TTL triggering of a SPECTRA X light engine. Two superimposed oscilloscope traces are shown in which the cyan intensity is adjusted from 100% to 55% via RS232 serial commands while the green intensity remains constant. Separation of the cyan and green pulses is ~0.25 ms.

Reference

[1] S Kubota, W Sidikejiang, K Seki et al. J Physiol(2019) 597:5025–5040

Download the PDF of Lumencor Light BYTES: May 2020

Light Reading: April 2020

Lumencor COVID-19 Update to Our Resellers

Lumencor remains open and operational with safety measures in place to ensure the health and productivity of our deeply committed team of professionals. We are ready and able to serve, advancing research and development with high-performance lighting specifically and precisely tailored to the instrumentation employed by those engaged in our fight against COVID-19. As an industry leader, we understand the critical role our products serve in the fight against this virus, and the many and varied others we seek to better understand and thereby control. Lumencor remains committed to supplying the global demand as safely, efficiently, and expeditiously as possible. We sincerely value our relationships with you. We thank you for your patience and unwavering support of our brand during this uncertain time. We remain available through your existing Lumencor contacts so please call upon us as your needs dictate.


WELCOME: Robert King, Global Director of Sales

We are pleased to welcome Robert King to the Lumencor team as Global Director of Sales. With a degree in neuroscience from the University of Cambridge, British-born Robert comes to us with extensive experience in life sciences through various roles he has held in sales, applications support, product management, and commercial leadership. Prior to joining Lumencor, Robert spent 10 years in leadership roles at Thermo Fisher Scientific, Danaher, and Carl Zeiss where he led technical sales teams and designed and managed sustainable and scalable commercial business processes.

In his new role with Lumencor, Robert’s mission is to build sustainable growth for our light engine business, launching in new markets and supporting our customers in the microscopy, materials science, clinical, and industrial manufacturing sectors. Robert is based out of the North San Francisco Bay Area.


Epi-illuminators for CELESTA light engines in Super-Resolution Imaging

Super-resolution imaging of RNA transcripts using multiplex error robust fluorescence in situ hybridization (MERFISH) and related methods in combination with the CELESTA light engine [1] requires higher levels of irradiance (mW/mm²) than can be obtained from Koehler illumination.  To facilitate these applications, Lumencor has developed a series of critical epi-illuminators that provide uniform illumination over a smaller field of view but with much higher irradiance than Koehler illumination.  Critical epi-illuminators compatible with all major research microscope brands are available.

Uniform fluorescent glass imaged with a CELESTA light engine coupled by an 800 µm diameter optical fiber to a critical epi-illuminator for Nikon Ti/Ti2 microscope. Image capture using a Nikon 60X/1.4 NA Plan Apo objective and an Andor Zyla 5.5 (2560 x 2160 pixels) sCMOS camera.  The plot shows the gray level values recorded by the camera along the diagonal axis marked in red.   The inset in the upper right-hand corner shows the same sample imaged with a Nikon 10X/0.3 NA Plan Apo objective.

Reference

[1] MA Wheeler, IC Clark, FJ Quintana et al. Nature (2020) 578:593–599


Independent Intensity and Pulse Width Control for Stroboscopic Illumination

Evaluation of photo-stimulation intensity dependence is often a necessary part of the analysis of neuromodulation resulting from optogenetic stimulation [1].  For this purpose, the pulse width and frequency of stroboscopic illumination (generated by TTL triggering), must be completely independent of intensity (pulse amplitude) settings applied via serial

commands.  As shown in the adjacent figure, the required control independence is exemplified in pulsed light output produced by the SPECTRA X light engine.  SPECTRA III, CELESTA, AURA, and RETRA light engines deliver similar performance in these applications. Alternating cyan (485/25 nm, ~0.5 ms) and green (560/32 nm, ~3 ms) output pulses generated by TTL triggering of a SPECTRA X light engine. Two superimposed oscilloscope traces are shown in which the cyan intensity is adjusted from 100% to 55% via RS232 serial commands while the green intensity remains constant.  Separation of the cyan and green pulses is ~0.25 ms.

Reference

[1] S Kubota, W Sidikejiang, K Seki et al. J Physiol(2019) 597:5025–5040


Lumencor Technical Trainings

In an effort to strengthen our partnerships with our resellers, Lumencor is currently offering a series of three technical training sessions on the entire Lumencor Product Portfolio:

1. White light sources (features, operation, and performance)

2. Color selective light sources (features, operation, and performance)

3. Applications of Lumencor light engines

Each training session is limited to one hour and is conducted remotely in a WebEx online meeting.  To schedule a technical training e-mail technical support specialist, Jasmin, at jasmin.schaefer@lumencor.com.

NOTE: To maintain qualified reseller status, resellers must sign up for the suite of technical trainings each calendar year.


Lumencor’s Earth Day Light Microscopy Imaging Competition

How did you celebrate the 50th anniversary of Earth Day this year?

At Lumencor, the celebration didn’t end on April 22nd, 2020. It continues on through June 29th as Lumencor’s Earth Day Light Microscopy Imaging Competition is in full swing, offering the opportunity to win up to $10,000 worth of state-of-the-art, solid-state lighting! Help us celebrate and promote a brighter, greener planet through the use of mercury-free illumination by submitting your images, acquired using Lumencor light engines, today. 

To find out more about the competition, click here.


Download the PDF of Light Reading: April 2020
lumencor
14940 NW Greenbrier Parkway | Beaverton, OR 97006 USA | 503.213.4269 | info@lumencor.com