Light BYTES – March 2020: LIDA Light Engine®, NIS Elements, and sCMOS Cameras for Color Light Microscopy
Lumencor’s Best Practices
Lumencor’s manufacturing operations remain healthy and active. While daily operations are inevitably constrained by measures taken to foster good health and minimize the transmission of COVID-19, productivity remains high. Minimal component shortages have developed as shelter-in-place practices have dampened our suppliers’ activities. However, we expect our supply chain will likely suffer additional shortages. Lumencor’s practices now include the quarantine of all receivables as our first commitment is to ensure the safety of our employees while safeguarding their work. In some cases, that may mean that our deliveries to customers will be delayed. We will do our best to inform all customers with pending orders of changes to anticipated shipment dates. Please notify us of any urgency around your order and we will do our very best to assist.
Please be assured that we are watching and managing your business with great care during the current emergency. Please contact to our sales, customer service or technical support representatives if you have any questions or wish to discuss deliverables for your organization’s needs.
A Spectacular Triple Play: LIDA Light Engine®, NIS Elements and sCMOS Cameras for Color Light Microscopy
For histologists, clinical pathologists and anyone seeking improvements in the speed, sensitivity and precision of transmitted light microscopy, Lumencor’s LIDA light engine in combination with Nikon NIS Elements software enable high-speed color imaging data without the need for a dedicated color camera. Instead, monochrome images generated by sequential triggering of the LIDA’s red, green and blue light sources by a sCMOS camera are processed by NIS Elements, delivering video-rate color output. These capabilities allow rapid and fully automated imaging of large tissue sections, as illustrated below. The software also enables convenient switching between camera-synchronized RGB illumination and white-light illumination for ocular viewing. Our application note RGB Color Imaging using the LIDA Light Engine and NIS Elements outlines hardware set-up for Hamamatsu ORCA-Flash4.0 and Andor Zyla sCMOS cameras and provides instructions for image acquisition control using NIS Elements software.
Color image of a 1.5 cm x 1 cm section of adenocarcinoma from human breast acquired using Lumencor’s LIDA light engine and NIS Elements software. Image courtesy of Dr. Michael Weber (Harvard Medical School).
Lumencor’s LIDA light engine mounted on the transillumination port of a Nikon Ti2 microscope with Andor Zyla 5.5 megapixel sCMOS camera
Submit your best microscopy images in Lumencor’s 2020 Earth Day Light Microscopy Competition
Winning submissions have the potential to earn you up to $10,000 worth of high tech, solid state lighting.