Light BYTES – September 2019: Take a Look at Our Winners from the 2019 Earth Day Light Microscopy Imaging Competition

SOLA® for the win!

This year Lumencor launched the Light Microscopy Imaging Competition in celebration of Earth Day to highlight Lumencor’s commitment to manufacturing bright, clean, and mercury-free light engines. We were impressed with the quality and breadth of applications in the submissions we received. Without further ado here are the winners for Lumencor’s 2019 Earth Day Light Microscopy Imaging Competition:

 

1st Place – Robert Zucker EPA, Research Triangle Park, NC PRIZE: NEW SOLA SE U-nIR Light engine

ARPE 19 cell treated with 75nm silver nanoparticles that are coated with PVP Combination of darkfield image (white nanoparticles) and fluorescence image of nuclei (blue), Golgi (green), and microtubules (red). Observation with a using a 60X plan Fluor lenses and a SOLA light engine®. The image was processed using Nikon deconvolution algorithms.


2nd Place – Glyn Nelson University of Newcastle, UK PRIZE: $1000 off voucher

Image of a mosquito larva’s ‘mustache’, the fine brush hairs on the side of their mouth. Z stack deconvolved and rendered in Huygens. Larva fixed in PFA and mounted in glycerol. Imaged with a Leica DMi8 equipped with a 40X oil 1.3 NA objective, GFP filter cube, Hamamatsu Flash4 v2 camera and a SOLA SE light engine.


3rd Place – Abdul Mohammed Ultivue, Cambridge, MA PRIZE: $500 off Voucher

16-plex biomarker profiling of Non-Small Cell Lung Cancer tissue (CD3, CD4, CD45RO, CD68, CD163, CD8, CD11c, CD20, PD1, PDL1, Cytokeratin, LAG3, FoxP3, Ki67, GranzymeB and MHCII). Nuclear counterstain shown in blue. The slide was processed using Ultivue’s InSituPlex technology and imaged on a fluorescence microscope using a 20X objective and SPECTRA X light engine®.

These images clearly demonstrate the quality of data attainable by using Lumencor’s solid state light engines. For more information on how Lumencor light engine can help you in you illumination needs please contact us.


Download the PDF of Light BYTES: September 2019

Packaging Guidance for Shipping Light Engines

Occasionally it will be necessary to ship a light engine back to our factory in Beaverton for service. On these occasions, you will receive an e-mail containing a return material authorization (RMA) and shipping instructions from our technical support department. Among those instructions are “Please ensure that the light engine is securely packaged for shipment, if possible in the original transit box in which it was delivered”. Secure and effective packaging is important in order to avoid additional costs in time and money for repairing damage incurred during transit. Here we offer some more detailed guidance on packaging light engines for shipment.

It is recommended to follow Lumencor’s packaging for new light engine shipments as closely as possible. The performance of this packaging has been proven in the course of thousands of shipments all over the world. An example is shown in the adjacent photo. Particularly important are the rigid expanded foam inserts. These prevent movement of the light engine within the box during transit. Bubble wrap, air pillows or loose packing material such as styrofoam “peanuts” are NOT effective substitutes and should NOT be used. Other important packaging tips include:

  • Detach the liquid light guide from the light engine and from its collimating adapter. Coil it loosely in the mylar pouch that it came in, or in a 2-gallon (33 x 38 cm) “ziploc” bag. Place the bag containing the light guide above the light engine in the shipping box, separated by a layer of packing material.
  • Do NOT use fibrous packing material that is liable to disintegrate during transit. Small pieces of packing material debris may end up deposited on the internal electronics and optics of the light engine.
  • Disconnect the DC power supply from the light engine and pack it separately inside the shipping box.

For additional guidance in preparing light engine shipments, please e-mail your questions to techsupport@lumencor.com.

Device Drivers for SPECTRA III, CELESTA, & AURA III light engines

The latest nightly builds of MicroManager 1.4.23 now include device drivers for SPECTRA III®, CELESTA® and AURA III® light engines. The basic set-up in the MicroManager Hardware Configuration Wizard is shown in the screenshot below.

Note that the GEN3 driver uses STANDARD mode communications. The light engine serial port addressed by MicroManager must therefore be set to STANDARD mode on the settings page of the Web GUI.

Other image acquisition software packages that now support SPECTRA III, CELESTA, and AURA III light engines are:

  • NIS Elements (Version 5.11 and above)
  • MetaMorph

For updates on controlling SPECTRA III, CELESTA, and AURA III light engines using other software packages, please email our Technical Support group at techsupport@lumencor.com.

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14940 NW Greenbrier Parkway | Beaverton, OR 97006 USA | 503.213.4269 | info@lumencor.com