power and intensity
Actual power output of specific unit subject to light engine design specification. Usable power a function of microscope maker, model. Estimated value ± 30%.
Bold Filter Designation: Lumencor standard, Tave > 90% for given bandpass
Asterisk “*”: Power and intensity estimates for FWHM bandpass
Download these Power & Intensity level tables [PDF]
light engine power, mW
| Color band | Fluor | Excitation Filter | 1mm fiber | 3mm LLG | Collimator |
|---|---|---|---|---|---|
| Violet (380-410nm) | DAPI/Hoechst | 386/23 379/34 390/18 395/25* | 60 50 80 80 | 150 130 200 250 | 175 150 250 275 |
| Blue (420-455nm) | CFP | 434/17 438/24 440/20* 445/20 | 85 105 70 60 | 230 275 200 160 | 280 350 250 190 |
| Cyan (460-490nm) | GFP/FITC | 470/24* 475/28 475/35 485/20 | 30 75 80 45 | 110 205 215 125 | 140 245 255 140 |
| Teal (500-520nm) | YFP | 510/10 510/25* 512/25 513/17 | 15 20 25 20 | 40 50 75 65 | 55 65 100 90 |
| Green (535-600nm) | TRITC/Cy3 | 542/27 549/15 549/21* 550/15* 560/25 | 110 95 65 50 100 | 390 260 210 190 380 | 705 695 590 515 865 |
| Yellow (535-600nm) | mCherry | 575/25 575/25* 586/20 585/29 | 100 55 75 80 | 300 210 200 210 | 660 600 650 650 |
| Red (620-750nm) | Cy5 | 628/40 632/22 640/30* 650/13 | 70 65 45 30 | 190 180 170 105 | 235 225 200 115 |
| NIR | 740/13 | 10 | 40 | 50 |
light engine intensity, mW/cm2
| Color Band | Fluor | Filter | 1mm fiber | 3mm LLG | Collimator |
|---|---|---|---|---|---|
| Violet (380-410nm) | DAPI/Hoechst | 386/23 379/34 390/18 395/25* | 7,640 6,370 10,200 10,200 | 2,120 1,840 2,830 3,540 | 36 31 51 56 |
| Blue (420-455nm) | CFP | 434/17 438/24 440/20* 445/20 | 10,800 13,400 8,920 7,640 | 3,250 3,890 2,830 2,260 | 57 71 51 39 |
| Cyan (460-490nm) | GFP/FITC | 470/24* 475/28 485/20 475/35 | 9,550 3,820 5,730 10,200 | 2,900 1,560 1,770 3,040 | 50 29 29 52 |
| Teal (500-520nm) | YFP | 510/10 510/25* 512/25 513/17 | 1,910 2,550 3,180 2,550 | 566 708 1,060 920 | 11 13 20 18 |
| Green (535-600nm) | TRITC/Cy3 | 542/27 549/15 549/21* 550/15* 560/25 | 14,000 12,100 8,280 6,370 12,700 | 5,520 3,680 2,970 2,690 5,380 | 144 142 120 105 176 |
| Yellow (535-600nm) | mCherry | 575/25 575/25* 586/20 585/29 | 12,732 7,000 9,550 10,186 | 4,250 2,970 2,830 2,970 | 134 122 132 132 |
| Red (620-750nm) | Cy5 | 628/40 632/22 640/30* 650/13 | 8,920 8,280 5,730 3,820 | 2,690 2,550 2,410 1,480 | 48 46 41 23 |
| NIR | 740/13 | 1270 | 570 | 10 |